caa a22 4500 445292946 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9263-9 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9263-9 Entry of bovine viral diarrhea virus into ovine cells occurs through clathrin-dependent endocytosis and low pH-dependent fusion [Elektronische Daten] [Basavaraj Mathapati, Niranjan Mishra, Katherukamem Rajukumar, Ram Nema, Sthita Behera, Shiv Dubey] Although mechanisms of bovine viral diarrhea virus (BVDV) entry into bovine cells have been elucidated, little is known concerning pestivirus entry and receptor usage in ovine cells. In this study, we determined the entry mechanisms of BVDV-1 and BVDV-2 in sheep fetal thymus cells. Both BVDV-1 and BVDV-2 infections were inhibited completely by chlorpromazine, β-methyl cyclodextrin, sucrose, bafilomycin A1, chloroquine, and ammonium chloride. Simultaneous presence of reducing agent and low pH resulted in marked loss of BVDV infectivity. Moreover, BVDV was unable to fuse with ovine cell membrane by the presence of reducing agent or low pH alone, while combination of both led to fusion at low efficiency. Furthermore, sheep fetal thymus cells acutely infected with BVDV-1 or BVDV-2 were found protected from heterologous BVDV infection. Taken together, our results showed for the first time that entry of both BVDV-1 and BVDV-2 into ovine cells occurred through clathrin-dependent endocytosis, endosomal acidification, and low pH-dependent fusion following an activation step, besides suggesting the involvement of a common ovine cellular receptor during attachment and entry. The Society for In Vitro Biology, 2009 Bovine viral diarrhea virus nationallicence Clathrin-dependent endocytosis nationallicence Endosomal acidification nationallicence Low pH-dependent fusion nationallicence Ovine cells nationallicence Mathapati Basavaraj High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut Mishra Niranjan High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut Rajukumar Katherukamem High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut Nema Ram High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut Behera Sthita High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut Dubey Shiv High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 403-407 1071-2690 46:5<403 2010 46 11626 https://doi.org/10.1007/s11626-009-9263-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9263-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mathapati Basavaraj High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Mishra Niranjan High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Rajukumar Katherukamem High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Nema Ram High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Behera Sthita High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 700 1- Dubey Shiv High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, 462021, Bhopal, Madhya Pradesh, India aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 403-407 1071-2690 46:5<403 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer