caa a22 4500 445292954 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s11626-009-9264-8 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9264-8 An effective method for the quantitative detection of porcine endogenous retrovirus in pig tissues [Elektronische Daten] [Peng Zhang, Ping Yu, Wei Wang, Li Zhang, Shengfu Li, Hong Bu] Xenotransplantation shows great promise for providing a virtually limitless supply of cells, tissues, and organs for a variety of therapeutical procedures. However, the potential of porcine endogenous retrovirus (PERV) as a human-tropic pathogen, particularly as a public health risk, is a major concern for xenotransplantation. This study focus on the detection of copy number in various tissues and organs in Banna Minipig Inbreed (BMI) from 2006 to 2007 in West China Hospital, Sichuan University. Real-time quantitative polymerase chain reaction (SYBR Green I) was performed in this study. The results showed that the pol gene had the most copy number in tissues compared with gag, envA, and envB. Our experiment will offer a rapid and accurate method for the detection of the copy number in various tissues and was especially suitable for the selection of tissues or organs in future clinical xenotransplantation. The Society for In Vitro Biology, 2010 PERV nationallicence Chinese pig nationallicence Real-time QPCR nationallicence Zhang Peng Department of Urology, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Yu Ping Laboratory of Cell and Gene Therapy, West China Second University Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Wang Wei Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Zhang Li Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Li Shengfu Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut Bu Hong Department of Pathology, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 408-410 1071-2690 46:5<408 2010 46 11626 https://doi.org/10.1007/s11626-009-9264-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9264-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Peng Department of Urology, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yu Ping Laboratory of Cell and Gene Therapy, West China Second University Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wei Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Li Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Li Shengfu Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Bu Hong Department of Pathology, West China Hospital, Sichuan University, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/5(2010-05-01), 408-410 1071-2690 46:5<408 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer