caa a22 4500 445292962 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9255-9 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9255-9 A simple in vitro culture system for tracheal cartilage development [Elektronische Daten] [Jinhyung Park, Jennifer Zhang, Ruth Choi, Irene Trinh, Peter Kim] Semi-circular tracheal cartilage is a critical determinant of maintaining architectural integrity of the respiratory airway. The current effort to understand the morphogenesis of tracheal cartilage is challenged by the lack of appropriate model systems. Here we report an in vitro tracheal cartilage system using embryonic tracheal-lung explants to recapitulate in vivo tracheal cartilage developmental processes. With modifications of a current lung culture protocol, we report a consistent in vitro technique of culturing tracheal cartilage from primitive mouse embryonic foregut for the first time. This tracheal culture system not only induces the formation of tracheal cartilage from the mouse embryonic foregut but also allows for the proper patterning of the developed tracheal cartilage. Furthermore, we show that this culture technique can be applied to culturing other types of cartilage in vertebrae, limbs, and ribs. We believe that this novel application of our in vitro culture system will facilitate the manipulation of cartilage development under various conditions and thus enabling us to advance our current limited knowledge on cartilage biology and development. The Society for In Vitro Biology, 2009 Trachea nationallicence Cartilage nationallicence Mouse nationallicence In vitro culture nationallicence Park Jinhyung Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut Zhang Jennifer Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut Choi Ruth Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut Trinh Irene Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut Kim Peter Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 92-96 1071-2690 46:2<92 2010 46 11626 https://doi.org/10.1007/s11626-009-9255-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9255-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Jinhyung Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Zhang Jennifer Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Choi Ruth Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Trinh Irene Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Kim Peter Department of Surgery and Program of Developmental Biology, The Hospital for Sick Children, 555 University Ave, Suite 1526, M5G 1X8, Toronto, ON, Canada aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 92-96 1071-2690 46:2<92 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer