caa a22 4500 445292970 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9247-9 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9247-9 Human colon tissue in organ culture: preservation of normal and neoplastic characteristics [Elektronische Daten] [Michael Dame, Narasimharao Bhagavathula, Cohra Mankey, Marissa DaSilva, Tejaswi Paruchuri, Muhammad Aslam, James Varani] Normal and neoplastic human colon tissue obtained at surgery was used to establish conditions for organ culture. Optimal conditions included an atmosphere of 5% CO2 and 95% O2; tissue partially submerged with mucosa at the gas interface; and serum-free medium with 1.5mM Ca2+ and a number of growth supplements. Histological, histochemical, and immunohistochemical features that distinguish normal and neoplastic tissue were preserved over a 2-d period. With normal tissue, this included the presence of elongated crypts with small, densely packed cells at the crypt base and mucin-containing goblet cells in the upper portion. Ki67 staining, for proliferating cells, was confined to the lower third of the crypt, while expression of extracellular calcium-sensing receptor was seen in the upper third and surface epithelium. E-cadherin and β-catenin were expressed throughout the epithelium and confined to the cell surface. In tumor tissue, the same disorganized, abnormal glandular structures seen at time zero were present after 2d. The majority of cells in these structures were mucin-poor, but occasional goblet cells were seen and mucin staining was present. Ki67 staining was seen throughout the abnormal epithelium and calcium-sensing receptor expression was weak and variable. E-cadherin was seen at the cell surface (similar to normal tissue), but in some places, there was diffuse cytoplasmic staining. Finally, intense cytoplasmic and nuclear β-catenin staining was observed in cultured neoplastic tissue. The Society for In Vitro Biology, 2009 Colon nationallicence Organ culture nationallicence Explant nationallicence Neoplasm nationallicence Calcium nationallicence Dame Michael The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut Bhagavathula Narasimharao The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut Mankey Cohra The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut DaSilva Marissa The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut Paruchuri Tejaswi The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut Aslam Muhammad The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut Varani James The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 114-122 1071-2690 46:2<114 2010 46 11626 https://doi.org/10.1007/s11626-009-9247-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9247-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dame Michael The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Bhagavathula Narasimharao The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Mankey Cohra The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- DaSilva Marissa The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Paruchuri Tejaswi The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Aslam Muhammad The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Varani James The Department of Pathology, University of Michigan Medical School, 48109, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 114-122 1071-2690 46:2<114 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer