caa a22 4500 445292989 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9250-1 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9250-1 The effects of various antioxidants on the development of parthenogenetic porcine embryos [Elektronische Daten] [Hyung Yuh, Dong Yu, Mi Shin, Hei Kim, Ki Bae, Dong Lee, Hwi Lee, Won Chang, Soo Park, Sang Lee, Hum Park, Ji Ha, Byung Hyun, Zae Ryoo] The major objective of this study was to improve the development rate of parthenogenetic porcine embryos. In this study, the anti-oxidative and anti-apoptotic effects of three antioxidants, β-mercaptoethanol (β-ME), α-tocopherol, and extracellular superoxide dismutase (EC-SOD), were examined on the development of parthenogenetic porcine embryos. The development rate of parthenogenetic porcine embryos to the blastocyst stage was 8.1% for control; 19.1%, 14.6%, and 5.0% for 1, 3, and 5μM β-ME; 17.2% and 17.5% for 50 and 100μM α-tocopherol and 12.0% and 4.0% for EC-SOD transgenic mouse embryonic fibroblast (Tg-MEF) and EC-SOD non-transgenic mouse embryonic fibroblast (NTg-MEF) conditioned medium at day 3, respectively. Here, β-ME, α-tocopherol, and EC-SOD Tg-MEF conditioned medium increased the development rate of parthenogenetic porcine embryos to the blastocyst stage (P < 0.05). The average number of total cells and apoptotic cells at the blastocyst was analyzed at the optimal conditions of the three antioxidants. The three antioxidants increased the average number of total cells at the blastocyst, and they decreased apoptotic cells at the blastocyst as compared to control without supplementation (P < 0.05). When the reactive oxygen species levels in two-cell embryos after 1μM β-ME and 100μM α-tocopherol treatment were examined, those were lower than control group (P < 0.05). In conclusion, it was found that the three antioxidants, β-mercaptoethanol, α-tocopherol, and EC-SOD Tg-MEF, conditioned medium can play a role as a strong stimulator in the development of parthenogenetic porcine embryos. The Society for In Vitro Biology, 2009 Parthenogenesis nationallicence Porcine nationallicence Embryo nationallicence Antioxidant nationallicence Apoptosis nationallicence Yuh Hyung School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Yu Dong School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Shin Mi School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Kim Hei School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Bae Ki School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Lee Dong School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Lee Hwi Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut Chang Won Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut Park Soo Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut Lee Sang School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Park Hum Department of Biotechnology, School of Engineering, Daegu University, 15 Jillyang, Gyeongsang, 712-714, Gyeongbuk, Republic of Korea aut Ha Ji School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut Hyun Byung Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangro, Yuseong-gu, 305-806, Daejeon, Republic of Korea aut Ryoo Zae School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 148-154 1071-2690 46:2<148 2010 46 11626 https://doi.org/10.1007/s11626-009-9250-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9250-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yuh Hyung School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Yu Dong School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Shin Mi School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Kim Hei School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Bae Ki School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Lee Dong School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Lee Hwi Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Chang Won Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Park Soo Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, 441-706, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Lee Sang School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Park Hum Department of Biotechnology, School of Engineering, Daegu University, 15 Jillyang, Gyeongsang, 712-714, Gyeongbuk, Republic of Korea aut NATIONALLICENCE 700 1- Ha Ji School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Hyun Byung Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangro, Yuseong-gu, 305-806, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Ryoo Zae School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 148-154 1071-2690 46:2<148 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer