caa a22 4500 445293004 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9246-x doi (NATIONALLICENCE)springer-10.1007/s11626-009-9246-x Hunter Wayne USDA, ARS, US Horticultural Research Laboratory, 2001 South Rock Road, 34945, Ft. Pierce, FL, USA aut Medium for development of bee cell cultures ( Apis mellifera [Elektronische Daten] Hymenoptera: Apidae) [Wayne Hunter] A media for the production of cell cultures from hymenopteran species such as honey bee, Apis mellifera L. (Hymenoptera: Apidae) was developed. Multiple bee cell cultures were produced when using bee larvae and pupae as starting material and modified Hert-Hunter 70 media. Cell culture systems for bees solves an impasse that has hindered efforts to isolate and screen pathogens which may be influencing or causing colony collapse disorder of bees. Multiple life stages of maturing larvae to early pupae were used to successfully establish cell cultures from the tissues of the head, thorax, and abdomen. Multiple cell types were observed which included free-floating suspensions, fibroblast-like, and epithelia-like monolayers. The final culture medium, WH2, was originally developed for hemipterans, Asian citrus psyllid, Diaphorina citri, and leafhopper, Homalodisca vitripennis cell cultures but has been shown to work for a diverse range of insect species such as bees. Bee cell cultures had various doubling times at 21-23°C ranging from 9-15d. Deformed wing virus was detected in the primary explanted tissues, which tested negative by rt-PCR for Israeli acute paralysis virus (IAPV), Kashmir bee virus, acute bee paralysis virus, and black queen cell virus. Culture inoculation with IAPV from an isolate from Florida field samples, was detectable in cell cultures after two subcultures. Cell culture from hymenoptera species, such as bees, greatly advances the approaches available to the field of study on colony collapse disorders. The Society for In Vitro Biology, 2009 Bee nationallicence Colony collapse disorder nationallicence CCD nationallicence Hymenoptera nationallicence Leafhopper nationallicence Psyllid nationallicence Virus nationallicence In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 83-86 1071-2690 46:2<83 2010 46 11626 https://doi.org/10.1007/s11626-009-9246-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9246-x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Hunter Wayne USDA, ARS, US Horticultural Research Laboratory, 2001 South Rock Road, 34945, Ft. Pierce, FL, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 83-86 1071-2690 46:2<83 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer