caa a22 4500 445293012 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9253-y doi (NATIONALLICENCE)springer-10.1007/s11626-009-9253-y Influence of rocky substrata on three-dimensional sponge cells model development [Elektronische Daten] [Marina Pozzolini, Laura Valisano, Carlo Cerrano, Mattia Menta, Stefano Schiaparelli, Giorgio Bavestrello, Umberto Benatti, Marco Giovine] Many marine and freshwater organisms are rocky bottom dwellers, and the mineralogical composition of the substratum is known to potentially condition their biology and ecology. In this work, we propose the use of 3D sponge cellular aggregates, called primmorphs, as suitable models for a multidisciplinary study of the mechanisms which regulate the biological responses triggered by the contact with different inorganic substrata. In our experiments, primmorphs obtained from the marine sponge Petrosia ficiformis (Poiret, 1789) were reared on calcium carbonate or on quartzitic substrata, respectively, and their morphological development was described. In parallel, the quantitative expression levels of two genes, silicatein and heat shock protein 70 (HSP70), were evaluated. The first gene is strictly correlated to spiculogenesis and sponge growth, while the second is an important indicator of stress. The results achieved with this in vitro model clearly demonstrate that quartzitic substrata determine the increase of silicatein gene expression, a lower expression of HSP70 gene, and a remarkable difference in primmorphs morphology compared to the analogous samples grown on marble. The Society for In Vitro Biology, 2009 Primmorphs nationallicence Silicatein nationallicence Marine sponges nationallicence Biomineralogy nationallicence Pozzolini Marina Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut Valisano Laura Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut Cerrano Carlo DIPTERIS, Università degli Studi di Genova, Corso Europa 26, 16132, Genova, Italy aut Menta Mattia DIMES-Sez. Biochimica, Università degli Studi di Genova, Viale Benedetto XV, 16132, Genova, Italy aut Schiaparelli Stefano DIPTERIS, Università degli Studi di Genova, Corso Europa 26, 16132, Genova, Italy aut Bavestrello Giorgio Dipartimento di Scienze del Mare, Università Politecnica delle Marche, 60131, Ancona, Italy aut Benatti Umberto DIMES-Sez. Biochimica, Università degli Studi di Genova, Viale Benedetto XV, 16132, Genova, Italy aut Giovine Marco Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 140-147 1071-2690 46:2<140 2010 46 11626 https://doi.org/10.1007/s11626-009-9253-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9253-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pozzolini Marina Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Valisano Laura Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Cerrano Carlo DIPTERIS, Università degli Studi di Genova, Corso Europa 26, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Menta Mattia DIMES-Sez. Biochimica, Università degli Studi di Genova, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Schiaparelli Stefano DIPTERIS, Università degli Studi di Genova, Corso Europa 26, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Bavestrello Giorgio Dipartimento di Scienze del Mare, Università Politecnica delle Marche, 60131, Ancona, Italy aut NATIONALLICENCE 700 1- Benatti Umberto DIMES-Sez. Biochimica, Università degli Studi di Genova, Viale Benedetto XV, 16132, Genova, Italy aut NATIONALLICENCE 700 1- Giovine Marco Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 140-147 1071-2690 46:2<140 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer