caa a22 4500 445293020 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9254-x doi (NATIONALLICENCE)springer-10.1007/s11626-009-9254-x Gene expression profiling of the developing mouse kidney and embryo [Elektronische Daten] [Lisa Shaw, Penny Johnson, Susan Kimber] The metanephros is formed from the reciprocal inductive interaction of two precursor tissues, the metanephric mesenchyme (MM) and the ureteric bud (UB). The UB induces MM to condense and differentiate forming the glomerulus and renal tubules, whilst the MM induces the UB to differentiate into the collecting tubules of the mature nephron. Uninduced MM is considered the progenitor cell population of the developing metanephros because of its potential to differentiate into more renal cell types than the UB. Previous studies have identified the phenotype of renal precursor cells; however, expression of candidate marker genes have not been analysed in other tissues of the murine embryo. We have assayed up to 19 candidate genes in eight embryonic tissues at five gestation stages of the mouse embryo to identify markers definitively expressed by renal cells during metanephric induction and markers developmentally regulated during kidney maturation. We then analysed their expression in other developing tissues. Results show Dcn, Hoxc9, Mest, Wt1 and Ywhaq were expressed at moderate to high levels during the window of metanephric specification and early differentiation (E10.5-E12.5 dpc), and Hoxc9, Ren1 and Wt1 expression was characteristic of mature renal cells. We demonstrated Cd24a, Cdh11, Mest, Scd2 and Sim2 were regulated during brain development, and Scd2, Cd24a and Sip1 expression was enriched in developing liver. These markers may be useful negative markers of kidney development. Use of a combination of highly expressed and negative markers may aid in the identification and removal of non-renal cells from heterogeneous populations of differentiating stem cells. The Society for In Vitro Biology, 2009 Metanephros nationallicence Embryo nationallicence Real-time quantitative PCR nationallicence Gene expression nationallicence Shaw Lisa Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton St, M13 9NT, Manchester, UK aut Johnson Penny Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Off Grafton Street, M13 9WL, Manchester, UK aut Kimber Susan Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton St, M13 9NT, Manchester, UK aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 155-165 1071-2690 46:2<155 2010 46 11626 https://doi.org/10.1007/s11626-009-9254-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9254-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shaw Lisa Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton St, M13 9NT, Manchester, UK aut NATIONALLICENCE 700 1- Johnson Penny Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Off Grafton Street, M13 9WL, Manchester, UK aut NATIONALLICENCE 700 1- Kimber Susan Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton St, M13 9NT, Manchester, UK aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 155-165 1071-2690 46:2<155 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer