caa a22 4500 445293055 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9248-8 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9248-8 Establishment and characterization of an osteopontin-null cutaneous squamous cell carcinoma cell line [Elektronische Daten] [Yu-Hua Hsieh, M. Juliana, Pi-Ling Chang] Osteopontin (OPN) is a secreted glycoprotein implicated to function in cancer development and metastasis. Although elevated expression of OPN are observed in cancer cells of various types, in some cases, only the cells in the stromal region surrounding the tumor express OPN, suggesting distinct functional roles for this protein derived from host cells and from cancer cells. To provide a model for addressing the functions and mechanisms of host-derived OPN in cancer progression and metastasis, a cutaneous squamous cell carcinoma cell line (ONSC) that lacks the OPN gene, Spp1, was established. This line of cells was derived from a squamous cell carcinoma that developed in a female, OPN-null mouse subjected to two-stage skin carcinogenesis. Morphologically, ONSC cells resemble epithelial cells, and they express the epithelial markers, K1, K14, and p63, as confirmed by immunohistochemical analyses. Genomic analyses indicate the presence of mutated H-Ras and p53 genes. ONSC cells form colonies in soft agar and, subcutaneously injected into athymic nude mice, develop into squamous cell carcinomas that metastasize to the lungs. Lacking OPN expression, these squamous cell carcinoma cells provide a model to address the function of host OPN in the context of cancer progression and metastasis. The Society for In Vitro Biology, 2009 Osteopontin nationallicence Cutaneous squamous cell carcinoma nationallicence Cell line nationallicence Tumor progression nationallicence Metastasis nationallicence Nude mice nationallicence Hsieh Yu-Hua Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, 35294, Birmingham, AL, USA aut Juliana M. Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA aut Chang Pi-Ling Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, 35294, Birmingham, AL, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 87-91 1071-2690 46:2<87 2010 46 11626 https://doi.org/10.1007/s11626-009-9248-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9248-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hsieh Yu-Hua Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, 35294, Birmingham, AL, USA aut NATIONALLICENCE 700 1- Juliana M. Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA aut NATIONALLICENCE 700 1- Chang Pi-Ling Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, 35294, Birmingham, AL, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 87-91 1071-2690 46:2<87 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer