caa a22 4500 445293071 CHVBK 20180317142534.0 cr unu---uuuuu 170323e20100201xx s 000 0 eng 10.1007/s11626-009-9258-6 doi (NATIONALLICENCE)springer-10.1007/s11626-009-9258-6 Equine bronchial epithelial cells differentiate into ciliated and mucus producing cells in vitro [Elektronische Daten] [Ute Schwab, M. Fulcher, Scott Randell, M. Flaminio, David Russell] We describe a method for creating differentiated equine bronchial epithelial cell cultures that can be used for in vitro studies including airway disease mechanisms and pathogen-host interactions. Our method is based on the culturing of human tracheobronchial epithelial cells at an air-liquid interface (ALI) in specific serum-free, hormone-supplemented medium. Bronchial epithelial cells are isolated and grown on T-Clear® insert membranes. Within 2 to 3wk, cells differentiate into ciliated and mucus producing cells as demonstrated by confocal and electron microscopy. Furthermore, the demonstration of the two major gel-forming mucin species, Muc5ac and Muc5b, in our bronchial epithelial cell culture system validates this method for studies of respiratory tract disease of the horse. The Society for In Vitro Biology, 2009 Bronchial epithelial cells nationallicence Horse nationallicence Cilia nationallicence Mucins nationallicence Schwab Ute Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut Fulcher M. Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC, USA aut Randell Scott Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC, USA aut Flaminio M. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut Russell David Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 102-106 1071-2690 46:2<102 2010 46 11626 https://doi.org/10.1007/s11626-009-9258-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-009-9258-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwab Ute Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Fulcher M. Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Randell Scott Cystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina, Chapel Hill, NC, USA aut NATIONALLICENCE 700 1- Flaminio M. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 700 1- Russell David Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/2(2010-02-01), 102-106 1071-2690 46:2<102 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer