caa a22 4500 445297867 CHVBK 20180317142547.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s12070-010-0111-8 doi (NATIONALLICENCE)springer-10.1007/s12070-010-0111-8 Role of Microsatellites Instability in Carcinogenesis of Postcricoid Carcinoma on Top of Plummer-Vinson Syndrome [Elektronische Daten] [Badawy Badawy, Mohamed Ahmad, Ramadan Sayed, Tito Habib] To develop a molecular pattern that might help in understanding carcinogenesis of postcricoid carcinoma (PCC) on top of Plummer-Vinson syndrome (PVS) in a prospective controlled study. Twenty-four patients with PVS were diagnosed and followed up over a 4year period, during which eight of them showed malignant change to PCC. Twenty volunteers free of neoplastic diseases were included as a control group. In the two groups, DNA extraction from mononuclear peripheral blood cells, and analysis of loss of heterozygosity (LOH) and microsatellite instability (MSI) using six paired simple tandem repeats (STRs) primers were done. The molecular weight of each STRs locus was scored and statistical correlations were performed. LOH occurred in 55.6 and 72.9% of PVS and PCC cases compared to 25% of control group. At loci D17S695, D9S753 and D9S171, LOH occurred in 54.2, 66.7, and 70.8% of PVS cases; and in 62.5% of PCC cases for each locus compared to 15, 25 and 45% of control cases. D3S1286 and CFS1-R displayed the highest frequency of LOH in PCC (100% for each) while recorded in 58.3 and 33.3% in PVS compared to 30 and 0% in control cases. Certain genetic events tend to occur as early and late events in malignant change of PVS to PCC. Detection of these events may help in understanding carcinogenesis and in early detection of malignancy. CFS1-R is the most informative marker of tumor progression. Association of Otolaryngologists of India, 2011 Plummer Vinson syndrome nationallicence Postcricoid carcinoma nationallicence Microsatellites instability nationallicence Badawy Badawy Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut Ahmad Mohamed Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut Sayed Ramadan Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut Habib Tito Department of Genetics, Sohag Faculty of Science, Sohag University, Sohag, Egypt aut Indian Journal of Otolaryngology and Head & Neck Surgery Springer-Verlag 62/4(2010-10-01), 417-420 0019-5421 62:4<417 2010 62 12070 https://doi.org/10.1007/s12070-010-0111-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12070-010-0111-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Badawy Badawy Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut NATIONALLICENCE 700 1- Ahmad Mohamed Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut NATIONALLICENCE 700 1- Sayed Ramadan Department of ENT, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt aut NATIONALLICENCE 700 1- Habib Tito Department of Genetics, Sohag Faculty of Science, Sohag University, Sohag, Egypt aut NATIONALLICENCE 773 0- Indian Journal of Otolaryngology and Head & Neck Surgery Springer-Verlag 62/4(2010-10-01), 417-420 0019-5421 62:4<417 2010 62 12070 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer