caa a22 4500 445298286 CHVBK 20180317142548.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s12013-010-9081-4 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9081-4 Construction of Small-Caliber, Polydiaxanone Cyclohexanone Vascular Stents [Elektronische Daten] [Qingjun You, Feng Wang, Liang Duan, Xiaodong Du, Mingdi Xiao, Zhenya Shen] Objective of this article is to construct and characterize a three-layered small-caliber, artificial vascular stent. The outer layer of the stent consisted of small intestine submucosa (SIS), the middle layer was the polydioxanone (PDS) vascular stents, and the inner layer. The SIS and PDS were attached with 8-0 PDS thread. Crosslinking with a 10% collagen/chondroitin sulfate solution and 0.020% glutaraldehyde secured the structure. The stent was implanted into the muscles on both sides of the canines' spine at 2, 4, 12, and 24weeks. A MTT assay using L-929 cells measured the cytotoxicity of the implant. Histological and microscopy analyses were employed to examine the degradation characteristics of the PDS stent. Biomechanical properties of the stent were tested and compared to those of normal physiological blood vessels. The PDS stent burst pressure (43.5±8.3) kPa, rupture intensity (19.1±1.56) N, strain ratio (42.88±3.16)%, and radial compliance (5.96±0.87)%/100mmHg were similar to that of physiological vessels. The cytotoxicity test showed that the PDS stent complied with specifications for biological materials for medical applications, with a cell toxicity ranging from 0 to 1. After 12weeks, SIS and collagen sponge were completely replaced by fibrous connective tissue. Although there was some degradation of PDS, inflammatory cell infiltration subsided. After 24weeks, the scaffold material began to absorb the new fibers and became filled with inflammatory cells and macrophages. This artificial vascular stent met the requirements of transplant experiments and should be further investigated for future clinical applications. Springer Science+Business Media, LLC, 2010 Tissue engineering nationallicence Small blood vessels nationallicence Polydioxanone cyclohexanone nationallicence Mechanical properties nationallicence Degradation nationallicence You Qingjun Cardiothoracic Surgery Department, Affiliated No. 4 Hospital, Suzhou University, 214062, Wu Xi, Jiang Su, People's Republic of China aut Wang Feng Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut Duan Liang Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut Du Xiaodong Cardiothoracic Surgery Department, Affiliated No. 4 Hospital, Suzhou University, 214062, Wu Xi, Jiang Su, People's Republic of China aut Xiao Mingdi Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut Shen Zhenya Cardiovascular Surgery Department, Affiliated No. 1 Hospital, Suzhou University, 215012, Suzhou, Jiang Su, People's Republic of China aut Cell Biochemistry and Biophysics Humana Press Inc 57/1(2010-05-01), 35-43 1085-9195 57:1<35 2010 57 12013 https://doi.org/10.1007/s12013-010-9081-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9081-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- You Qingjun Cardiothoracic Surgery Department, Affiliated No. 4 Hospital, Suzhou University, 214062, Wu Xi, Jiang Su, People's Republic of China aut NATIONALLICENCE 700 1- Wang Feng Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Duan Liang Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Du Xiaodong Cardiothoracic Surgery Department, Affiliated No. 4 Hospital, Suzhou University, 214062, Wu Xi, Jiang Su, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Mingdi Department of Cardiovascular Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Shen Zhenya Cardiovascular Surgery Department, Affiliated No. 1 Hospital, Suzhou University, 215012, Suzhou, Jiang Su, People's Republic of China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 57/1(2010-05-01), 35-43 1085-9195 57:1<35 2010 57 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer