caa a22 4500 445298294 CHVBK 20180317142548.0 cr unu---uuuuu 170323e20100501xx s 000 0 eng 10.1007/s12013-010-9076-1 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9076-1 Static Magnetic Fields Inhibit Proliferation and Disperse Subcellular Localization of Gamma Complex Protein3 in Cultured C2C12 Myoblast Cells [Elektronische Daten] [SeungChan Kim, Wooseok Im] Magnetic fields may delay the rate of cell cycle progression, and there are reports that magnetic fields induce neurite outgrowth in cultured neuronal cells. To demonstrate whether magnetic field also effects on myoblast cells in cell growth, C2C12 cell lines were cultured and 2000G static magnetic field was applied. After 48h of incubation, both the WST-1 assay (0.01<P<0.025, t-test) and direct cell counting (P<0.0005, t-test) showed that static magnetic fields inhibit the proliferation of cultured C2C12 cells. Immunocytochemistry for alpha and tubulin gamma complex protein (TUBA and GCP3) was made and applying a static magnetic field-dispersed tubulin GCP3 formation, a intracellular apparatus for tubulin structuring in cell division. This protein expression was not altered by western blot. This study indicates that applying a static magnetic field alters the subcellular localizing of GCP3, and may delay the cell growth in cultured C2C12 myoblast cells. Springer Science+Business Media, LLC, 2010 Cell proliferation nationallicence Dispersion nationallicence Gamma complex protein nationallicence Magnets nationallicence Kim SeungChan Department of Biotechnology, Yonsei University, Seoul, Korea aut Im Wooseok Clinical Research Institute, Seoul National University Hospital Neuroscience Lab, Seoul, Korea aut Cell Biochemistry and Biophysics Humana Press Inc 57/1(2010-05-01), 1-8 1085-9195 57:1<1 2010 57 12013 https://doi.org/10.1007/s12013-010-9076-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9076-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim SeungChan Department of Biotechnology, Yonsei University, Seoul, Korea aut NATIONALLICENCE 700 1- Im Wooseok Clinical Research Institute, Seoul National University Hospital Neuroscience Lab, Seoul, Korea aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 57/1(2010-05-01), 1-8 1085-9195 57:1<1 2010 57 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer