caa a22 4500 445298340 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20100701xx s 000 0 eng 10.1007/s12013-010-9085-0 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9085-0 Spontaneous Rhythmic Inward Currents Recorded in Interstitial Cells of Rabbit Portal Vein [Elektronische Daten] [Xu Huang, Dan Zhao, Zuo-Yu Wang, Ming-Liang Zhang, Zhi-Qiang Yan, Yan-Fei Han, Hong-Li Lu, Wen-Xie Xu, Zong-Lai Jiang] It is now well established that smooth muscle of the portal vein exhibits spontaneous rhythmic contraction in vitro. The present study was designed to investigate the pacemaking mechanism(s) underlying the spontaneous rhythmic contractions in the rabbit portal vein (RPV). Using whole-cell patch clamp techniques, spontaneous inward currents were recorded at −60mV of holding potential in freshly dispersed c-Kit immunopositive interstitial cells (ICs) isolated from sections of RPV. The inward currents were abolished by caffeine, FCCP, thapsigargin, and ryanodine, but were partially inhibited by 2-APB. Both gadolinium, a non-selective cation channel inhibitor, and niflumic acid, a chloride channel blocker, inhibited the inward currents completely. Replacement of external Na+ with NMDG+ also blocked the inward currents. W-7, a calmodulin inhibitor, increased both the amplitude and frequency of the inward currents. Taken together, these results indicate that non-selective cationic channels are involved in the generation of spontaneous inward currents recorded from ICs. Intracellular calcium concentration and calmodulin regulate the spontaneous inward currents, which may account for spontaneous rhythmic contraction in the RPV, but a role of chloride channels may not be excluded in the present study. Springer Science+Business Media, LLC, 2010 Spontaneous inward currents nationallicence Interstitial cells nationallicence Rabbit portal vein nationallicence Huang Xu Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut Zhao Dan Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut Wang Zuo-Yu Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut Zhang Ming-Liang Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut Yan Zhi-Qiang Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut Han Yan-Fei Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut Lu Hong-Li Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut Xu Wen-Xie Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut Jiang Zong-Lai Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut Cell Biochemistry and Biophysics Humana Press Inc 57/2-3(2010-07-01), 77-85 1085-9195 57:2-3<77 2010 57 12013 https://doi.org/10.1007/s12013-010-9085-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9085-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Xu Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Zhao Dan Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Wang Zuo-Yu Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Ming-Liang Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Yan Zhi-Qiang Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Han Yan-Fei Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Lu Hong-Li Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Xu Wen-Xie Department of Physiology, School of Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Jiang Zong-Lai Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang, P.O. Box 888, 200240, Shanghai, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 57/2-3(2010-07-01), 77-85 1085-9195 57:2-3<77 2010 57 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer