caa a22 4500 445298383 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s12013-010-9091-2 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9091-2 α-Mangostin Suppresses Phorbol 12-myristate 13-acetate-Induced MMP-2/MMP-9 Expressions via αvβ3 Integrin/FAK/ERK and NF-κB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells [Elektronische Daten] [Yuan-Wei Shih, Shang-Tao Chien, Pin-Shern Chen, Jian-Hui Lee, Shu-Hau Wu, Li-Te Yin] The purpose of this study is to investigate the anti-metastatic effect of α-mangostin on phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions in A549 human lung adenocarcinoma cells. Firstly, α-mangostin could inhibit PMA-induced abilities of the adhesion, invasion, and migration. Data also showed α-mangostin could inhibit the activation of αvβ3 integrin, focal adhesion kinase (FAK), and extracellular signal-regulated kinase1/2 (ERK1/2) involved in the downregulation the enzyme activities, protein and messenger RNA levels of MMP-2 and MMP-9 induced by PMA. Next, α-mangostin also strongly inhibited PMA-induced degradation of inhibitor of kappaBα (IκBα) and the nuclear levels of nuclear factor kappa B (NF-κB). Also, a dose-dependent inhibition on the binding abilities of NF-κB by α-mangostin treatment was further observed. Furthermore, reduction of FAK or ERK1/2 phosphorylation by FAK small interfering RNA (FAK siRNA) potentiated the effect of α-mangostin. Finally, the transient transfection of ERK siRNA significantly down-regulated the expressions of MMP-2 and MMP-9 concomitantly with a marked inhibition on cell invasion and migration. Presented results indicated α-mangostin is a novel, effect, anti-metastatic agent that functions by downregulating MMP-2 and MMP-9 gene expressions. Springer Science+Business Media, LLC, 2010 α-Mangostin nationallicence PMA nationallicence Invasion nationallicence Migration nationallicence αvβ3 Integrin nationallicence FAK nationallicence ERK1/2 nationallicence MMP-2 nationallicence MMP-9 nationallicence PMA : Phorbol 12-myristate 13-acetate nationallicence MMPs : Matrix metalloproteinases nationallicence ECM : Extracellular matrix nationallicence ERK : Extracellular signaling-regulating kinase nationallicence JNK/SAPK : c-Jun N-terminal kinase/stress-activated protein kinase nationallicence p38 MAPK : p38 Mitogen-activated protein kinase nationallicence PI3K/Akt : Phosphoinositide 3-kinase/protein kinase B nationallicence NF-κB : Nuclear factor kappa B nationallicence IκBα : Inhibitor of NF-κB nationallicence Shih Yuan-Wei Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut Chien Shang-Tao Department of Pathology, Kaohsiung Armed Forces General Hospital, No. 2, Chung-Zhang, 1st Road, 802, Kaohsiung, Taiwan aut Chen Pin-Shern Department of Biological Science and Technology, Chia Nan University of Pharmacy & Science, No. 60, Section1, Erh-Jen Road, Jen-Te, 717, Tainan, Taiwan aut Lee Jian-Hui Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut Wu Shu-Hau Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut Yin Li-Te Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut Cell Biochemistry and Biophysics Humana Press Inc 58/1(2010-09-01), 31-44 1085-9195 58:1<31 2010 58 12013 https://doi.org/10.1007/s12013-010-9091-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9091-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shih Yuan-Wei Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut NATIONALLICENCE 700 1- Chien Shang-Tao Department of Pathology, Kaohsiung Armed Forces General Hospital, No. 2, Chung-Zhang, 1st Road, 802, Kaohsiung, Taiwan aut NATIONALLICENCE 700 1- Chen Pin-Shern Department of Biological Science and Technology, Chia Nan University of Pharmacy & Science, No. 60, Section1, Erh-Jen Road, Jen-Te, 717, Tainan, Taiwan aut NATIONALLICENCE 700 1- Lee Jian-Hui Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut NATIONALLICENCE 700 1- Wu Shu-Hau Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut NATIONALLICENCE 700 1- Yin Li-Te Department of Biological Science and Technology and Graduate Institute of Biomedical Science, Chung Hwa University of Medical Technology, No. 89, Wen-Hwa 1st Street, Jen-Te, 717, Tainan, Taiwan aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/1(2010-09-01), 31-44 1085-9195 58:1<31 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer