caa a22 4500 445298391 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s12013-010-9086-z doi (NATIONALLICENCE)springer-10.1007/s12013-010-9086-z Hypothesis: Human Umbilical Cord Blood-derived Stromal Cells Promote Megakaryocytopoiesis Through the Influence of SDF-1 and PECAM-1 [Elektronische Daten] [Yimei Feng, Xinghua Chen, Lei Gao] The development and maturation of megakaryocytes (MKs) is a complex and multistage cellular and biological process. The hematopoietic microenvironment plays an important role in megakaryocytopoiesis regulation. Stromal cells, an important ingredient in the hematopoietic microenvironment, may regulate the development of MKs via the adhesion with MKs and via augmentation of cytokine secretion. Our laboratory has previously isolated a novel population of stromal cells from human umbilical cord blood, called hUCBDSCs. Compared with hBMSCs, the hUCBDSCs express higher levels of stromal cell-derived factor-1 (SDF-1) and increase the colony-forming-unit-megakaryocytes (CFU-MK). Meanwhile, there are reports identified a migration defect in PECAM-1-deficient MKs in response to a gradient of SDF-1. Based on literature searches and our experimental findings, we present a hypothesis that hUCBDSCs, secreting high level of SDF-1, modulated the expression of PECAM-1 of MKs, to regulate the megakaryocyte development. Springer Science+Business Media, LLC, 2010 Hematopoietic microenvironment nationallicence Umbilical cord blood nationallicence Stromal cells nationallicence Megakaryocytopoiesis nationallicence Stromal cell-derived factor-1 nationallicence Platelet endothelial cell adhesion molecule-1 nationallicence Feng Yimei Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut Chen Xinghua Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut Gao Lei Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut Cell Biochemistry and Biophysics Humana Press Inc 58/1(2010-09-01), 25-30 1085-9195 58:1<25 2010 58 12013 https://doi.org/10.1007/s12013-010-9086-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9086-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Yimei Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut NATIONALLICENCE 700 1- Chen Xinghua Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut NATIONALLICENCE 700 1- Gao Lei Department of Hematology, Second Affiliated Hospital, Third Military Medical University, 400037, Chongqing, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/1(2010-09-01), 25-30 1085-9195 58:1<25 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer