caa a22 4500 445298421 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12013-010-9104-1 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9104-1 Effects of Menadione, Hydrogen Peroxide, and Quercetin on Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T-Cells [Elektronische Daten] [Irina Baran, Constanta Ganea, Agata Scordino, Francesco Musumeci, Vincenza Barresi, Salvatore Tudisco, Simona Privitera, Rosaria Grasso, Daniele Condorelli, Ioan Ursu, Virgil Baran, Eva Katona, Maria-Magdalena Mocanu, Marisa Gulino, Raluca Ungureanu, Mihaela Surcel, Cornel Ursaciuc] Menadione (MD) is an effective cytotoxic drug able to produce intracellularly large amounts of superoxide anion. Quercetin (QC), a widely distributed bioflavonoid, can exert both antioxidant and pro-oxidant effects and is known to specifically inhibit cell proliferation and induce apoptosis in different cancer cell types. We have investigated the relation between delayed luminescence (DL) induced by UV-laser excitation and the effects of MD, hydrogen peroxide, and QC on apoptosis and cell cycle in human leukemia Jurkat T-cells. Treatments with 500μM H2O2 and 250μM MD for 20min produced 66.0±4.9 and 46.4±8.6% apoptotic cell fractions, respectively. Long-term (24h) pre-exposure to 5μM, but not 0.5μM QC enhanced apoptosis induced by MD, whereas short-term (1h) pre-incubation with 10μM QC offered 50% protection against H2O2-induced apoptosis, but potentiated apoptosis induced by MD. Since physiological levels of QC in the blood are normally less than 10μM, these data can provide relevant information regarding the benefits of flavonoid-combined treatments of leukemia. All the three drugs exerted significant effects on DL. Our data are consistent with (1) the involvement of Complex I of the mitochondrial respiratory chain as an important source of delayed light emission on the 10μs-10ms scale, (2) the ability of superoxide anions to quench DL on the 100μs-10ms scale, probably via inhibition of reverse electron transfer at the Fe/S centers in Complex I, and (3) the relative insensitivity of DL to intracellular OH• and H2O2 levels. Springer Science+Business Media, LLC, 2010 Apoptosis nationallicence Delayed luminescence nationallicence Oxidative stress nationallicence Flavonoids nationallicence Mitochondrial respiratory chain nationallicence Leukemia nationallicence Baran Irina Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut Ganea Constanta Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut Scordino Agata Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Musumeci Francesco Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Barresi Vincenza Dipartimento di Scienze Chimiche, Sezione di Biochimica e Biologia Molecolare, Università di Catania, 6 A. Doria, 95125, Catania, Italy aut Tudisco Salvatore Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Privitera Simona Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Grasso Rosaria Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Condorelli Daniele Dipartimento di Scienze Chimiche, Sezione di Biochimica e Biologia Molecolare, Università di Catania, 6 A. Doria, 95125, Catania, Italy aut Ursu Ioan IFIN-HH, 407 Atomistilor Str., 077125 Magurele, Bucharest, Romania aut Baran Virgil IFIN-HH, 407 Atomistilor Str., 077125 Magurele, Bucharest, Romania aut Katona Eva Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut Mocanu Maria-Magdalena Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut Gulino Marisa Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut Ungureanu Raluca Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut Surcel Mihaela Department of Immunology, "Victor Babes” National Institute, 99-101 Spl. Independentei, Bucharest, Romania aut Ursaciuc Cornel Department of Immunology, "Victor Babes” National Institute, 99-101 Spl. Independentei, Bucharest, Romania aut Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 169-179 1085-9195 58:3<169 2010 58 12013 https://doi.org/10.1007/s12013-010-9104-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9104-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baran Irina Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut NATIONALLICENCE 700 1- Ganea Constanta Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut NATIONALLICENCE 700 1- Scordino Agata Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Musumeci Francesco Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Barresi Vincenza Dipartimento di Scienze Chimiche, Sezione di Biochimica e Biologia Molecolare, Università di Catania, 6 A. Doria, 95125, Catania, Italy aut NATIONALLICENCE 700 1- Tudisco Salvatore Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Privitera Simona Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Grasso Rosaria Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Condorelli Daniele Dipartimento di Scienze Chimiche, Sezione di Biochimica e Biologia Molecolare, Università di Catania, 6 A. Doria, 95125, Catania, Italy aut NATIONALLICENCE 700 1- Ursu Ioan IFIN-HH, 407 Atomistilor Str., 077125 Magurele, Bucharest, Romania aut NATIONALLICENCE 700 1- Baran Virgil IFIN-HH, 407 Atomistilor Str., 077125 Magurele, Bucharest, Romania aut NATIONALLICENCE 700 1- Katona Eva Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut NATIONALLICENCE 700 1- Mocanu Maria-Magdalena Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut NATIONALLICENCE 700 1- Gulino Marisa Laboratori Nazionali del Sud, Istituto Nazionale di Fisica Nucleare, 62 S. Sofia, Catania, Italy aut NATIONALLICENCE 700 1- Ungureanu Raluca Department of Biophysics, "Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474, Bucharest, Romania aut NATIONALLICENCE 700 1- Surcel Mihaela Department of Immunology, "Victor Babes” National Institute, 99-101 Spl. Independentei, Bucharest, Romania aut NATIONALLICENCE 700 1- Ursaciuc Cornel Department of Immunology, "Victor Babes” National Institute, 99-101 Spl. Independentei, Bucharest, Romania aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 169-179 1085-9195 58:3<169 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer