caa a22 4500 445298464 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12013-010-9098-8 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9098-8 The Mechanism and Characterization of Learning and Memory Impairment in Sleep-Deprived Mice [Elektronische Daten] [Zhi-Qiang Xu, Chang-Yue Gao, Chuan-Qin Fang, Hua-Dong Zhou, Xiao-Jiang Jiang] Objectives are to examine the effects of sleep deprivation (SD) on spatial learning and memory in mice, to determine how SD effects the expression of phosphorylated cyclic AMP responsive element binding protein (pCREB) in mouse hippocampus, and to explore the mechanism of influence of sleep deprivation on cognitive function. Twenty, 3-month-old female C57BL/6J mice were randomly assigned into two groups, the sleep deprivation group (SD, n=10) and control group with normal sleep (CC, n=10). The mice in SD group were deprived sleep by "gentle touch” for 20days and then all the mice were subjected for Morris Water Maze test to determine the mean latency of escape (LE). Percentage of time spent in the target quadrant was calculated. Mouse hippocampus pCREB levels were quantified by western blot. Compared with CC group, SD mice had a significantly longer mean LE time (P<0.05) and spent less time in the target quadrant (P<0.05). Western blot revealed that hippocampus pCREB levels in the SD group were significantly lower than that in control group (0.71±0.03 vs 0.82±0.06, P<0.01). The impairment in spatial learning and memory in sleep-deprived animals may be associated with the reduction of pCREB in the hippocampus. Springer Science+Business Media, LLC, 2010 Sleep deprivation nationallicence Learning nationallicence Memory nationallicence pCREB nationallicence Xu Zhi-Qiang Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut Gao Chang-Yue Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut Fang Chuan-Qin Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut Zhou Hua-Dong Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut Jiang Xiao-Jiang Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 137-140 1085-9195 58:3<137 2010 58 12013 https://doi.org/10.1007/s12013-010-9098-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9098-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Zhi-Qiang Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Gao Chang-Yue Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Fang Chuan-Qin Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Zhou Hua-Dong Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Jiang Xiao-Jiang Department of Neurology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, No. 10 Changjiang Branch Road, Daping, 400042, Chongqing, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 137-140 1085-9195 58:3<137 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer