caa a22 4500 445298472 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12013-010-9099-7 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9099-7 Diabetes-Related Alteration of Occludin Expression in Rat Blood-Spinal Cord Barrier [Elektronische Daten] [Yuwu Zhao, Xueyuan Liu, Aiyong Yu, Yajun Zhou, Bangjian Liu] Occludin is an essential component of tight junctions, which are involved in controlling the integrity of the blood-brain barrier and blood-spinal cord barrier (BSCB). Diabetes-induced alteration of occludin in rat BSCB and the relationship between occludin level and disease course was examined. Diabetes was induced using streptozotocin. Occludin rat spinal cord mRNA levels were assessed by real-time quantitative RT-PCR. Protein levels were examined by western blot. Occludin expression in 1-month diabetic rats was significantly reduced compared to the controls (0.20±0.01 vs 1.00±0.01, respectively; P<0.05). Expression was also significantly lower in the 3-month diabetic group (0.06±0.02; P<0.01). Occludin protein levels of 1-month (0.53±0.01) and 3-month (0.31±0.01) diabetic rats were also significantly reduced compared to controls (0.91±0.06; P<0.01 for both). Diabetes decreased BSCB occludin expression at the mRNA and protein level. This down-regulation appears to correlate with the course of the disease, and may be a causal factor of diabetes-induced increase of BSCB permeability. Springer Science+Business Media, LLC, 2010 Diabetes nationallicence Blood-spinal cord barrier nationallicence Tight junctions nationallicence Occludin nationallicence BBB : Blood-brain barrier nationallicence BSCB : Blood-spinal cord barrier nationallicence BRB : Blood-retina barrier nationallicence CNS : Central nervous system nationallicence ZO-1 : Zonula occludens-1 nationallicence MMP : Matrix metalloproteinase nationallicence HIV : Human immunodeficiency virus nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence Zhao Yuwu Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut Liu Xueyuan Department of Neurology, Shanghai Tongji University Affiliated Tenth People's Hospital, 301 Yanchang Road, 200072, Shanghai, People's Republic of China aut Yu Aiyong Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut Zhou Yajun Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut Liu Bangjian Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 141-145 1085-9195 58:3<141 2010 58 12013 https://doi.org/10.1007/s12013-010-9099-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9099-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Yuwu Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xueyuan Department of Neurology, Shanghai Tongji University Affiliated Tenth People's Hospital, 301 Yanchang Road, 200072, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yu Aiyong Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Yajun Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Bangjian Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 141-145 1085-9195 58:3<141 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer