caa a22 4500 445298499 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12013-010-9103-2 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9103-2 Crystal Structure of the Protein l -Isoaspartyl Methyltransferase from Escherichia coli [Elektronische Daten] [Pengfei Fang, Xu Li, Jing Wang, Li Xing, Yan Gao, Liwen Niu, Maikun Teng] Among the known covalent damages that can occur spontaneously to proteins, the formation of isoaspartyl linkages through deamidation of asparagines and isomerization of aspartates may be one of the most rapid forms under conditions of physiological pH and temperature. The protein l-isoaspartyl methyltransferase (PIMT) is thought to recognize l-isoaspartyl residues and repair this kind of damaged proteins. Curiously, there is a potential functional difference between bacterial and mammalian PIMTs. Herein, we present the crystal structure of Escherichia coli PIMT (EcPIMT) at a resolution of 1.8Å. The enzyme we investigated was able to remain bound to its product S-adenosylhomocysteine (SAH) during crystallization. Analysis indicates that the high affinity of EcPIMT for SAH might lead to the lower activity of the enzyme. Springer Science+Business Media, LLC, 2010 Protein damage nationallicence PIMT nationallicence Repair and degradation nationallicence Crystal structure nationallicence Methyltransferase nationallicence Fang Pengfei Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Li Xu Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Wang Jing Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Xing Li Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Gao Yan Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Niu Liwen Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Teng Maikun Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 163-167 1085-9195 58:3<163 2010 58 12013 https://doi.org/10.1007/s12013-010-9103-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9103-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fang Pengfei Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Li Xu Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jing Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Xing Li Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yan Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Niu Liwen Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 700 1- Teng Maikun Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, 230026, Hefei, Anhui, People's Republic of China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 163-167 1085-9195 58:3<163 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer