caa a22 4500 445298502 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12013-010-9102-3 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9102-3 Plasma Factor in Red Blood Cells Adhesion to Endothelial Cells: Humans and Rats [Elektronische Daten] [Avraham Schlager, Gideon Zamir, Gregory Barshtein, Saul Yedgar, Dan Arbell] Erythrocyte adhesion to the vascular endothelium is one of the key determinants of microcirculatory blood flow. Adhesion is a complex process determined by the intricate interaction among red blood cells (RBC), plasma factors, and the vascular endothelium. Rats are commonly used as disease models to investigate the pathophysiology of various hematological disease processes occurring in humans and their response to prospective treatments. The aim of our study was to characterize the adhesion of RBC in adult blood from rat and human subjects, in order to test the validity of rat models for adhesion-related disease processes. We demonstrated that adhesion of RBC from rats (rRBC), to endothelial cells (EC) in plasma-free buffer, is stronger than from human subjects (hRBC). In addition, plasma proteins induced elevation of hRBC (eightfold) but depression of rRBC (threefold) adhesion to EC. It is thus suggested to be aware of the difference in RBC/EC interaction for human and rat subjects, when studying models of blood flow. Springer Science+Business Media, LLC, 2010 Erythrocyte adhesion nationallicence Blood plasma nationallicence Animal model nationallicence Schlager Avraham Surgery Department, New York University Hospital, New York, NY, USA aut Zamir Gideon Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel aut Barshtein Gregory Department of Biochemistry, The Faculty of Medicine, The Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut Yedgar Saul Department of Biochemistry, The Faculty of Medicine, The Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut Arbell Dan Pediatric Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel aut Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 157-161 1085-9195 58:3<157 2010 58 12013 https://doi.org/10.1007/s12013-010-9102-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9102-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schlager Avraham Surgery Department, New York University Hospital, New York, NY, USA aut NATIONALLICENCE 700 1- Zamir Gideon Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel aut NATIONALLICENCE 700 1- Barshtein Gregory Department of Biochemistry, The Faculty of Medicine, The Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Yedgar Saul Department of Biochemistry, The Faculty of Medicine, The Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Arbell Dan Pediatric Surgery Department, Hadassah-Hebrew University Medical Center, Jerusalem, Israel aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/3(2010-12-01), 157-161 1085-9195 58:3<157 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer