caa a22 4500 445298510 CHVBK 20180317142549.0 cr unu---uuuuu 170323e20101101xx s 000 0 eng 10.1007/s12013-010-9096-x doi (NATIONALLICENCE)springer-10.1007/s12013-010-9096-x Changes in Proteomic Features Induced by Insulin on Vascular Smooth Muscle Cells from Spontaneous Hypertensive Rats In Vitro [Elektronische Daten] [Yan Wang, Bo Zhang, Yun Bai, Chunyu Zeng, Xukai Wang] Hyperinsulinemia is a risk factor in atherosclerosis formation that it stimulated vascular smooth muscle cells (VSMCs) proliferation and migration. To understand the underlying molecular mechanism involved in the processes of cellular response to insulin, VSMCs from Wistar-Kyoto rat (WKY) and spontaneous hypertensive rat (SHR) were isolated and cultured, and its proteome was comparatively analyzed with normal control by two-dimensional gel electrophoresis (2-DE). Results showed that the proliferation of VSMCs from SHR be more sensitive to insulin stimulation than that VSMCs from WKY. The detectable spots ranged from 537 to 608 on the gels in VSMCs of SHR, and 413±31 spots in VSMCs of WKY. The different expressed protein spots in VSMCs of SHR were then isolated and measured by matrix-assisted desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). A total of 18 spots showed a sharp clear spectrum, and 13 spots matched with the known proteins from database. These proteins were mainly involved in cytoskeleton, glycometabolism, and post-translational processes. Among these proteins, OPN and matrix gla protein were up-regulated expression proteins, while α-SM actin was down-regulated. Furthermore, these preliminarily identified proteins confirmed by RT-PCR and western blotting analysis were coincident with the changes in 2-DE check. In addition, the cytoskeleton changes and migration rate of VSMCs from SHR treated by insulin increased significantly. The results showed that insulin plays a crucial role in activating proliferation and migration of VSMCs, by regulating the phenotype switch of VSMCs. Springer Science+Business Media, LLC, 2010 Comparative proteomics nationallicence Insulin nationallicence VSMC phenotype nationallicence α-SM actin nationallicence Osteopontin nationallicence Matrix gla protein nationallicence VSMC : Vascular smooth muscle cells nationallicence 2-DE : Two-dimensional gel electrophoresis nationallicence MALDI-TOF-MS : Matrix-assisted desorption/ionization time of flight mass spectrometry nationallicence SHR : Spontaneous hypertensive rat nationallicence WKY : Wistar-Kyoto rat nationallicence α-SM actin : α-Smooth muscle actin nationallicence OPN : Osteopontin nationallicence Wang Yan Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut Zhang Bo Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut Bai Yun Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut Zeng Chunyu Department of Cardiovascular Disease, Daping Hospital, Third Military Medical University, Daping Avenue No 10, 400042, Chongqing, China aut Wang Xukai Department of Cardiovascular Disease, Daping Hospital, Third Military Medical University, Daping Avenue No 10, 400042, Chongqing, China aut Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 97-106 1085-9195 58:2<97 2010 58 12013 https://doi.org/10.1007/s12013-010-9096-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9096-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yan Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Zhang Bo Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Bai Yun Department of Medical Genetics, Third Military Medical University, 400038, Chongqing, China aut NATIONALLICENCE 700 1- Zeng Chunyu Department of Cardiovascular Disease, Daping Hospital, Third Military Medical University, Daping Avenue No 10, 400042, Chongqing, China aut NATIONALLICENCE 700 1- Wang Xukai Department of Cardiovascular Disease, Daping Hospital, Third Military Medical University, Daping Avenue No 10, 400042, Chongqing, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 97-106 1085-9195 58:2<97 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer