caa a22 4500 445298545 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20101101xx s 000 0 eng 10.1007/s12013-010-9095-y doi (NATIONALLICENCE)springer-10.1007/s12013-010-9095-y Effect of Bone Morphogenetic Protein 4 in the Human Brain Glioma Cell Line U251 [Elektronische Daten] [Baohui Liu, Daofeng Tian, Wei Yi, Liquan Wu, Qiang Cai, Huimin Dong, Heng Shen, Baowei Ji, Long Wang, Shenqi Zhang, Dong Ruan, Qianxue Chen] The role of bone morphogenetic protein 4 (BMP4) in gliomas is not clear. We hypothesized that BMP4 inhibits proliferation in the brain glioma cell line U251 through a signaling pathway involving BMP4 and the mothers against decapentaplegic homolog 4 (SMAD4) protein. We exposed U251 cells to Adriamycin (1g) for 48h; cell proliferation (MTT assay), expression of BMP4 and SMAD4 (mRNA: qPCR; protein: Western blot) were studied. We further altered expression of BMP4 by overexpression or siRNA silencing, and documented cell responses to Adriamycin. Proliferation of U251 cells was significantly inhibited upon exposure to Adriamycin. This inhibition was associated with increased expression of BMP4. Further, proliferation of U251 cells was inhibited when BMP4 was overexpressed. BMP4 expression negatively correlated with expression of SMAD4, such that elevated levels of BMP4 were associated with decreased expression of SMAD4 and vice versa. The Adriamycin-induced inhibition of proliferation of U251 cells was attenuated when BMP4 was knocked down by siRNA. To conclude, BMP4 is associated with inhibition of proliferation of U251 cells; the effects of BMP4 involve the BMP4-Smad signaling pathway. BMP4 has a potential as a target for glioma therapy. Springer Science+Business Media, LLC, 2010 Bone morphogenetic protein 4 nationallicence Mothers against decapentaplegic homolog 4 nationallicence Target therapy nationallicence U251 nationallicence Adriamycin nationallicence Liu Baohui Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Tian Daofeng Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Yi Wei Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Wu Liquan Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Cai Qiang Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Dong Huimin Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Shen Heng Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Ji Baowei Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Wang Long Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Zhang Shenqi Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Ruan Dong Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Chen Qianxue Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 91-96 1085-9195 58:2<91 2010 58 12013 https://doi.org/10.1007/s12013-010-9095-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9095-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Baohui Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Tian Daofeng Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Yi Wei Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wu Liquan Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Cai Qiang Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Dong Huimin Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Shen Heng Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Ji Baowei Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Wang Long Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhang Shenqi Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Ruan Dong Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Chen Qianxue Renmin Hospital, Wuhan University, 238 Jiefang Street, 430060, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 91-96 1085-9195 58:2<91 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer