caa a22 4500 445298553 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20101101xx s 000 0 eng 10.1007/s12013-010-9090-3 doi (NATIONALLICENCE)springer-10.1007/s12013-010-9090-3 Redox Buffer Capacity of the Cell: Theoretical and Experimental Approach [Elektronische Daten] [Grigory Martinovich, Irina Martinovich, Sergey Cherenkevich, Heinrich Sauer] Reactive oxygen species (ROS) are involved in a variety of biological phenomena, such as mutation, carcinogenesis, inflammation, aging, development, and signal transduction. Intracellular generation of ROS might lead to the activation of redox signaling or oxidative stress. Nonetheless, it is difficult to estimate whether ROS-induced intracellular events are beneficial or deleterious to the cell. The quantitative basis of changes in the intracellular redox state of cells is not well-defined, thus leading to the dilemma that redox changes induced by oxidants in distinct cell types cannot be predicted. To overcome this limitation this study undertakes to analyze on a theoretical as well as on an experimental basis the intracellular redox state changes occurring inside cells upon addition of oxidants or reductants. 2,7-Dichlorodihydrofluorescein (H2DCF) was used to characterize the redox buffer capacity in erythrocytes. It was shown that the redox buffer capacity of erythrocytes in the relation to peroxynitrite (ONOO−) is 2.1times lower than the redox buffer capacity of erythrocytes in the relation to hydrogen peroxide (H2O2). The feasibility of redox buffer capacity assessment as an innovative tool for investigation and description of redox signaling events in cells is discussed. Springer Science+Business Media, LLC, 2010 Reactive oxygen species nationallicence Redox state nationallicence Reduction potential nationallicence Redox signaling nationallicence Oxidative stress nationallicence Nernst equation nationallicence Martinovich Grigory Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut Martinovich Irina Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut Cherenkevich Sergey Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut Sauer Heinrich Institute of Physiology, Aulweg 129, 35392, Giessen, Germany aut Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 75-83 1085-9195 58:2<75 2010 58 12013 https://doi.org/10.1007/s12013-010-9090-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-010-9090-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martinovich Grigory Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut NATIONALLICENCE 700 1- Martinovich Irina Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut NATIONALLICENCE 700 1- Cherenkevich Sergey Department of Biophysics, Belarusian State University, Nesavisimosti Ave., 4, 220030, Minsk, Republic of Belarus aut NATIONALLICENCE 700 1- Sauer Heinrich Institute of Physiology, Aulweg 129, 35392, Giessen, Germany aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 58/2(2010-11-01), 75-83 1085-9195 58:2<75 2010 58 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer