caa a22 4500 445298588 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s12013-009-9069-0 doi (NATIONALLICENCE)springer-10.1007/s12013-009-9069-0 Activation of p38 MAPK Pathway by Hepatitis C Virus E2 in Cells Transiently Expressing DC-SIGN [Elektronische Daten] [Qiu-Li Chen, Shi-Ying Zhu, Zhong-Qi Bian, Lan-Juan Zhao, Jie Cao, Wei Pan, Zhong-Tian Qi] Dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a cellular receptor for hepatitis C virus for the binding of viral envelope glycoprotein E2. Interaction of DC-SIGN with the E2 may evoke cellular signal transduction implicated in viral pathogenesis. We developed a cell model with DC-SIGN transient transfection to study p38 mitogen-activated protein kinase (MAPK) signaling pathway in response to the E2 treatment. HEK293T and HeLa were DC-SIGN-deficient cell lines. DC-SIGN was detectable at the surface of HEK293T and HeLa transfected with DC-SIGN, and the levels of DC-SIGN were high in transfected-HEK293T as compared with HeLa. The transfected-HEK293T displayed ability for the E2 binding. In the transfected-HEK293T, level of p38 MAPK phosphorylation was increased upon the E2 treatment and reduced following blockage of DC-SIGN with an antibody against DC-SIGN. Phosphorylation of downstream transcription factor activating transcription factor (ATF)-2 was also up-regulated by the E2 via DC-SIGN. Similar results were obtained with NIH3T3 cells stably expressing DC-SIGN and Huh7 cells. Our results indicate that DC-SIGN transient expression in HEK293T is a useful cell model for investigating p38 MAPK pathway triggered by the E2, which may provide information for understanding cellular receptors-mediated signaling events and the viral pathogenesis. Humana Press Inc., 2009 DC-SIGN nationallicence p38 MAPK nationallicence Hepatitis C virus nationallicence E2 nationallicence Receptor nationallicence Chen Qiu-Li Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Zhu Shi-Ying Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Bian Zhong-Qi Center for Infectious Diseases, CPLA Kunming General Hospital, 650032, Kunming, China aut Zhao Lan-Juan Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Cao Jie Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Pan Wei Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Qi Zhong-Tian Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut Cell Biochemistry and Biophysics Humana Press Inc 56/1(2010-01-01), 49-58 1085-9195 56:1<49 2010 56 12013 https://doi.org/10.1007/s12013-009-9069-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-009-9069-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Qiu-Li Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Zhu Shi-Ying Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Bian Zhong-Qi Center for Infectious Diseases, CPLA Kunming General Hospital, 650032, Kunming, China aut NATIONALLICENCE 700 1- Zhao Lan-Juan Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Cao Jie Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Pan Wei Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 700 1- Qi Zhong-Tian Department of Microbiology, CPLA Key Laboratory of Medical Microbiology, Second Military Medical University, 800 Xiang Yin Road, 200433, Shanghai, China aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 56/1(2010-01-01), 49-58 1085-9195 56:1<49 2010 56 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer