caa a22 4500 445298618 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s12013-009-9075-2 doi (NATIONALLICENCE)springer-10.1007/s12013-009-9075-2 Probing Cell Structure Responses Through a Shear and Stretching Mechanical Stimulation Technique [Elektronische Daten] [Robert Steward Jr., Chao-Min Cheng, Danny Wang, Philip LeDuc] Cells are complex, dynamic systems that respond to various in vivo stimuli including chemical, mechanical, and scaffolding alterations. The influence of mechanics on cells is especially important in physiological areas that dictate what modes of mechanics exist. Complex, multivariate physiological responses can result from multi-factorial, multi-mode mechanics, including tension, compression, or shear stresses. In this study, we present a novel device based on elastomeric materials that allowed us to stimulate NIH 3T3 fibroblasts through uniaxial strip stretching or shear fluid flow. Cell shape and structural response was observed using conventional approaches such as fluorescent microscopy. Cell orientation and actin cytoskeleton alignment along the direction of applied force were observed to occur after an initial 3h time period for shear fluid flow and static uniaxial strip stretching experiments although these two directions of alignment were oriented orthogonal relative to each other. This response was then followed by an increasingly pronounced cell and actin cytoskeleton alignment parallel to the direction of force after 6, 12, and 24h, with 85% of the cells aligned along the direction of force after 24h. These results indicate that our novel device could be implemented to study the effects of multiple modes of mechanical stimulation on living cells while probing their structural response especially with respect to competing directions of alignment and orientation under these different modes of mechanical stimulation. We believe that this will be important in a diversity of fields including cell mechanotransduction, cell-material interactions, biophysics, and tissue engineering. Springer Science+Business Media, LLC, 2009 Cell morphology nationallicence Cell alignment nationallicence Actin filaments nationallicence Polydimethylsiloxane nationallicence NIH 3T3 fibroblasts nationallicence Uniaxial strip stretch nationallicence Shear fluid flow nationallicence Steward Jr. Robert Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Cheng Chao-Min Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Wang Danny Institute of Biomedical Science, Academia Sinica, 11529, Taipei, Taiwan aut LeDuc Philip Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 115-124 1085-9195 56:2-3<115 2010 56 12013 https://doi.org/10.1007/s12013-009-9075-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-009-9075-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steward Jr Robert Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Cheng Chao-Min Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Wang Danny Institute of Biomedical Science, Academia Sinica, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- LeDuc Philip Departments of Mechanical and Biomedical Engineering and Biological Sciences, Carnegie Mellon University, 420 Scaife Hall, 5000 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 115-124 1085-9195 56:2-3<115 2010 56 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer