caa a22 4500 445298626 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s12013-009-9072-5 doi (NATIONALLICENCE)springer-10.1007/s12013-009-9072-5 On Some Aspects of the Thermodynamic of Membrane Recycling Mediated by Fluid Phase Endocytosis: Evaluation of Published Data and Perspectives [Elektronische Daten] [Cyril Rauch, Alain Pluen, Neil Foster, Paul Loughna, Ali Mobasheri, Dominique Lagadic-Gossmann, Laurent Counillon] The theoretical and experimental description of fluid phase endocytosis (FPE) requires an asymmetry in phospholipid number between the two leaflets of the cell membrane, which provides the biomechanical torque needed to generate membrane budding. Although the motor force behind FPE is defined, its kinetic has yet to be determined. Based on a body of evidences suggesting that the mean surface tension is unlikely to be involved in endocytosis we decided to determine whether the cytosolic hydrostatic pressure could be involved, by considering a constant energy exchanged between the cytosol and the cell membrane. The theory is compared to existing experimental data obtained from FPE kinetic studies in living cells where altered phospholipid asymmetry or changes in the extracellular osmotic pressure have been investigated. The model demonstrates that FPE is dependent on the influx and efflux of vesicular volumes (i.e. vesicular volumes recycling) rather than the membrane tension of cells. We conclude that: (i) a relationship exists between membrane lipid number asymmetry and resting cytosolic pressure and (ii) the validity of Laplace's law is limited to cells incubated in a definite hypotonic regime. Finally, we discuss how the model could help clarifying elusive observations obtained from different fields and including: (a) the non-canonical shuttling of aquaporin in cells, (b) the relationship between high blood pressure and inflammation and (c) the mechanosensitivity of the sodium/proton exchanger. Springer Science+Business Media, LLC, 2009 Endocytosis nationallicence Exocytosis nationallicence Aminophospholipid translocase nationallicence Osmotic pressure nationallicence Phospholipid nationallicence Rauch Cyril School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut Pluen Alain School of Pharmacy and Pharmaceutical Science, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut Foster Neil School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut Loughna Paul School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut Mobasheri Ali School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut Lagadic-Gossmann Dominique EA 4427 SeRAIC, Université Rennes I, IFR 140, 2 avenue du Professeur Leon-Bernard, 35043, Rennes Cedex, France aut Counillon Laurent CNRS FRE3093 Transport ionique aspects normaux et pathologiques, Faculté des Sciences, Université de Nice-Sophia Antipolis, Parc Valrose, 06108, Nice Cedex 2, France aut Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 73-90 1085-9195 56:2-3<73 2010 56 12013 https://doi.org/10.1007/s12013-009-9072-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-009-9072-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rauch Cyril School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut NATIONALLICENCE 700 1- Pluen Alain School of Pharmacy and Pharmaceutical Science, University of Manchester, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Foster Neil School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut NATIONALLICENCE 700 1- Loughna Paul School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut NATIONALLICENCE 700 1- Mobasheri Ali School of Veterinary Medicine and Science, University of Nottingham, College Road, LE12 5RD, Sutton Bonington, UK aut NATIONALLICENCE 700 1- Lagadic-Gossmann Dominique EA 4427 SeRAIC, Université Rennes I, IFR 140, 2 avenue du Professeur Leon-Bernard, 35043, Rennes Cedex, France aut NATIONALLICENCE 700 1- Counillon Laurent CNRS FRE3093 Transport ionique aspects normaux et pathologiques, Faculté des Sciences, Université de Nice-Sophia Antipolis, Parc Valrose, 06108, Nice Cedex 2, France aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 73-90 1085-9195 56:2-3<73 2010 56 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer