caa a22 4500 445298634 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s12013-009-9071-6 doi (NATIONALLICENCE)springer-10.1007/s12013-009-9071-6 Secreted Phosphoprotein 1 Upstream Invasive Network Construction and Analysis of Lung Adenocarcinoma Compared with Human Normal Adjacent Tissues by Integrative Biocomputation [Elektronische Daten] [Ying Sun, Lin Wang, Minghu Jiang, Juxiang Huang, Zhenqiu Liu, Stefan Wolfl] The aim of this study is to set up single molecular secreted phosphoprotein 1 (SPP1) upstream invasive network of lung adenocarcinoma. This paper proposed an integrated method based on linear programming and a decomposition procedure with integrated analysis of the significant function cluster using Kappa statistics and fuzzy heuristic clustering. Our study proved that only modules appearing in lung adenocarcinoma include cytokine module (CXCL13, GREM1_2 inhibition), cell adhesion module (COL11A1_2 activation; CDH3 inhibition), and receptor binding module (NMU activation; CXCL13, GREM1_2 inhibition), which increase the invasion of cancer cell. We compared skeletal development, signal, biological regulation, sequence variant modules between human normal adjacent tissues and lung adenocarcinoma. SPP1 skeletal development module appears in human normal adjacent tissues (COL11A1_1 activation; COL10A1 inhibition), whereas in lung adenocarcinoma (COL11A1_2, COL1A2 activation); signal module appears in human normal adjacent tissues (COL11A1_1, CXCL13, MMP11, SPINK1 activation; COL10A1, COL3A1 inhibition), whereas in lung adenocarcinoma (COL11A1_2, COL1A2, MMP12 activation; CDH3, CXCL13, GREM1_2, MMP11, SPINK1 inhibition); biological regulation module appears in human normal adjacent tissues (CXCL13, MKI67, PYCR1 activation; NEK2, SPDEF, TOP2A_2, TOX3_1 inhibition), whereas in lung adenocarcinoma (HMGB3, MKI67, NMU, PYCR1, TOX3_2 activation; CXCL13, SPDEF, TOP2A_2 inhibition); sequence variant module appears in human normal adjacent tissues (COL11A1_1, MKI67, MMP11 activation; ASPM, COL10A1, COL3A1, NEK2, TMPRSS4, TOP2A_2 inhibition), whereas in lung adenocarcinoma (COL11A1_2, COL1A2, HMMR, MKI67, MMP12 activation; ABCC3, ASPM, CDH3, MMP11, TOP2A_2 inhibition). It can be deduced that modules above in human normal adjacent tissues reflect the invasive inhibition of normal cells, whereas in lung adenocarcinoma increase the invasion of cancer cell. Our study of SPP1 upstream invasive network may be useful to identify novel and potentially targets for prognosis and therapy of lung adenocarcinoma. Springer Science+Business Media, LLC, 2009 SPP1 upstream invasive network construction nationallicence Function analysis nationallicence Human lung adenocarcinoma nationallicence Integrative biocomputation nationallicence GENE_SYMBOL : Gene name nationallicence COL3A1 : Collagen, type iii, alpha 1 (ehlers-danlos syndrome type iv, autosomal) nationallicence COL10A1 : Collagen, type x, alpha 1 (schmid metaphyseal chondrodysplasia) nationallicence TOP2A : Topoisomerase (dna) ii alpha 170kDa nationallicence ABCC3 : ATP-binding cassette, sub-family c (cftr/mrp), member 3 nationallicence COL11A1 : Collagen, type xi, alpha 1 nationallicence TMPRSS4 : Type ii membrane serine protease nationallicence GREM1 : Gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis) nationallicence PYCR1 : Pyrroline-5-carboxylate reductase 1 nationallicence ASPM : Pypothetical protein flj10517 nationallicence HMMR : Hyaluronan-mediated motility receptor (rhamm) nationallicence COL1A2 : Collagen, type i, alpha 2 nationallicence SPPI : Secreted phosphoprotein 1 (osteopontin, bone sialoprotein i, early tlymphocyte activation 1) nationallicence SPINK1 : Serine peptidase inhibitor, kazal type 1 nationallicence NMU : Neuromedin u nationallicence CDH3 : Cadherin 3, type 1, p-cadherin (placental) nationallicence HMGB3 : High-mobility group box 3 nationallicence NEK2 : Nima (never in mitosis gene a)-related kinase 2 nationallicence TOX3 : Trinucleotide repeat containing 9 nationallicence MKI67 : Antigen identified by monoclonal antibody ki-67 nationallicence SPDEF : Sam pointed domain containing ets transcription factor nationallicence MMP12 : Matrix metallopeptidase 12 (macrophage elastase) nationallicence CXCL13 : Chemokine (c-x-c motif) ligand 13 (b-cell chemoattractant) nationallicence MMP11 : Matrix metallopeptidase 11 (stromelysin 3) nationallicence Sun Ying Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut Wang Lin Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut Jiang Minghu Lab of Computational Linguistics, School of Humanities and Social Sciences, Tsinghua University, 100084, Beijing, China aut Huang Juxiang Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut Liu Zhenqiu Department of Epidemiology and Preventive Medicine, University of Maryland Medicine, Baltimore, USA aut Wolfl Stefan Department of Biology-Bioanalytic, Institute of Pharmacia & Molecular Biotechnology, University of Heidelberg, 69120, Heidelberg, Germany aut Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 59-71 1085-9195 56:2-3<59 2010 56 12013 https://doi.org/10.1007/s12013-009-9071-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-009-9071-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Ying Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut NATIONALLICENCE 700 1- Wang Lin Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut NATIONALLICENCE 700 1- Jiang Minghu Lab of Computational Linguistics, School of Humanities and Social Sciences, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Huang Juxiang Biomedical Center, School of Electronics Engineering, Beijing University of Posts and Telecommunications, 100876, Beijing, China aut NATIONALLICENCE 700 1- Liu Zhenqiu Department of Epidemiology and Preventive Medicine, University of Maryland Medicine, Baltimore, USA aut NATIONALLICENCE 700 1- Wolfl Stefan Department of Biology-Bioanalytic, Institute of Pharmacia & Molecular Biotechnology, University of Heidelberg, 69120, Heidelberg, Germany aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 59-71 1085-9195 56:2-3<59 2010 56 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer