caa a22 4500 445298650 CHVBK 20180317142550.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s12013-009-9074-3 doi (NATIONALLICENCE)springer-10.1007/s12013-009-9074-3 Vitamin E Induces Phosphatidylserine Externalization and Red Cell Adhesion to Endothelial Cells [Elektronische Daten] [Alexander Koshkaryev, Gregory Barshtein, Saul Yedgar] Red blood cell (RBC) adhesion to vessel wall endothelium is a potent catalyst of vascular occlusion and occurs in oxidative stress states such as hemoglobinopathies and cardiovascular conditions. These are often treated with vitamin E (VitE), a "classic” antioxidant. In this study, we examined the effects of VitE on RBC adhesion to vascular endothelial cells (EC), and on translocation of phosphatidylserine (PS) to RBC surface, known as a potent mediator of RBC/EC adhesion, facilitating thrombus formation. Treatment of RBC with VitE strongly induces (up to sevenfold) PS externalization and enhances (up to 20-fold) their adherence to EC. The VitE hydrophilic analogue—Trolox—does not incorporate into cell membranes. Trolox did not exhibit any of these effects, implying that the VitE effect is due to its known ability to incorporate into cell membranes. The membrane-incorporated VitE significantly reduced the level of reactive oxygen species in H2O2-treated RBC, demonstrating that VitE elevates RBC/EC adhesion despite acting as an anti-oxidant. This study demonstrates for the first time that contrary to the common view of VitE as a beneficial supplement, VitE may introduce a circulatory risk by inducing flow-disturbing RBC adherence to blood vessel wall and the pro-thrombotic PS exposure. Springer Science+Business Media, LLC, 2009 α-Tocopherol nationallicence Red blood cells nationallicence Adherence nationallicence Endothelial cells nationallicence Phosphatidylserine nationallicence Koshkaryev Alexander Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut Barshtein Gregory Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut Yedgar Saul Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 109-114 1085-9195 56:2-3<109 2010 56 12013 https://doi.org/10.1007/s12013-009-9074-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12013-009-9074-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koshkaryev Alexander Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Barshtein Gregory Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Yedgar Saul Department of Biochemistry, Hebrew University-Hadassah Medical School, 91120, Jerusalem, Israel aut NATIONALLICENCE 773 0- Cell Biochemistry and Biophysics Humana Press Inc 56/2-3(2010-04-01), 109-114 1085-9195 56:2-3<109 2010 56 12013 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer