caa a22 4500 445301597 CHVBK 20180317142559.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s13237-010-0002-0 doi (NATIONALLICENCE)springer-10.1007/s13237-010-0002-0 Cytomixis-like chromosomes/chromatin elimination from pollen mother cells (PMCs) in wheat-rye allopolyploids [Elektronische Daten] [Anna Kalinka, Magdalena Achrem, Stanisława Rogalska] Polyploidy is an important event and major force in plant speciation. Amongst the polyploids, allopolyploids have attracted special attention to investigate genetic and epigenetic mechanisms. Also, they are the means for the development of new genotypes and genomic combinations to facilitate genetic enhancement and agricultural productivity. Whereas natural allopolyploids are genetically stable and well adapted, the newly synthesized ones are highly unstable. This instability is manifested into alterations at genomic and/or phenotypic level. Here we present the phenomenon of direct chromosome/chromatin elimination from pollen mother cells (PMCs) in wheat-rye hybrids as one aspect of instability leading to irregular meiosis and disturbances in meiotic process. One of the prominent irregularities noticed is peripherally separated uncondensed or pycnotic masses of chromatin in all meiotic stages. We have observed that this chromatin undergoes elimination by budding-like way, whereby a "mini-cell” is created. It was also found that nucleoli are the first to be eliminated along with a small mass of chromatin. By means of GISH we have shown that both rye and wheat chromatin might be eliminated. In the separated groups of chromosomes/chromatin neither DNaseI nor DNase II activity was detected. Immunolocalization of tubulin allowed for differentiation between chromatin elimination from microspores and elimination from earlier stages of meiosis. It was noticeable, that in microspores special cytoskeleton structure pushing micronuclei out from the cells was created. Elimination occurred before and after meiosis as well as in each stage of meiotic division, but its intensity varied, depending on the PMC. The basis of the elimination mechanism might be the same as in cytomixis, because both phenomena share common symptoms, although cytomixis per se was rare in the analyzed hybrids. Archana Sharma Foundation of Calcutta, 2010 Wheat-rye nationallicence Meiosis PMC nationallicence Chromosome/chromatine elimination nationallicence Cytomixis-like mechanism nationallicence Kalinka Anna Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut Achrem Magdalena Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut Rogalska Stanisława Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut The Nucleus Springer-Verlag 53/3(2010-12-01), 69-83 0029-568X 53:3<69 2010 53 13237 https://doi.org/10.1007/s13237-010-0002-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13237-010-0002-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kalinka Anna Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut NATIONALLICENCE 700 1- Achrem Magdalena Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut NATIONALLICENCE 700 1- Rogalska Stanisława Cell Biology, University of Szczecin, ul. Waska 13, 71-415, Szczecin, Poland aut NATIONALLICENCE 773 0- The Nucleus Springer-Verlag 53/3(2010-12-01), 69-83 0029-568X 53:3<69 2010 53 13237 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer