caa a22 4500 445301600 CHVBK 20180317142559.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s13237-011-0016-2 doi (NATIONALLICENCE)springer-10.1007/s13237-011-0016-2 Standardized karyotype of the three-spot gourami, Trichogaster trichopterus (Perciformes, Belontidae) from Thailand by conventional and Ag-NOR staining technique [Elektronische Daten] [Weerayuth Supiwong, Alongklod Tanomtong, Anan Kenthao, Kriengkrai Seetapan, Sarawut Kaewsri, La-orsri Sanoamuang] Standardized karyotype and idiogram of the three-spot gourami (Trichogaster trichopterus) from local market in Roi-Et province, northeast Thailand, were studied. Kidney cell samples were taken from ten male and ten female fishes. The mitotic chromosome preparations were prepared by blood cell culture as well as directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosome number of T. trichopterus was 2n = 46, the fundamental numbers (NF) were 46 in both male and female. The types of autosomes were 12 large telocentric, 32 medium telocentric and 2 small telocentric chromosomes. The region adjacent to the telomere of chromosome pair 2 showed clearly observable secondary constriction / NORs. The karyotype formula for T. trichopterus could be deduced as: $$ {2}n\left( {\hbox{diploid}} \right){46} = {\hbox{L}}_{{12}}^{\rm{t}} + {\hbox{M}}_{{32}}^{\rm{t}} + {\hbox{S}}_2^{\rm{t}} $$. Archana Sharma Foundation of Calcutta, 2011 Three-spot gourami nationallicence Trichogaster trichopterus nationallicence Ag-NOR staining nationallicence Karyotype nationallicence Supiwong Weerayuth Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut Tanomtong Alongklod Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut Kenthao Anan Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut Seetapan Kriengkrai School of Agriculture and Natural Resources, Naresuan University Phayao, Tumbol Maeka, 56000, Muang, Phayao, Thailand aut Kaewsri Sarawut Program in Applied Biology, Department of Science, Faculty of Science, Buriram Rajabhat University, 31000, Muang, Buriram, Thailand aut Sanoamuang La-orsri Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut The Nucleus Springer-Verlag 53/3(2010-12-01), 103-107 0029-568X 53:3<103 2010 53 13237 https://doi.org/10.1007/s13237-011-0016-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13237-011-0016-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Supiwong Weerayuth Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Tanomtong Alongklod Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Kenthao Anan Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Seetapan Kriengkrai School of Agriculture and Natural Resources, Naresuan University Phayao, Tumbol Maeka, 56000, Muang, Phayao, Thailand aut NATIONALLICENCE 700 1- Kaewsri Sarawut Program in Applied Biology, Department of Science, Faculty of Science, Buriram Rajabhat University, 31000, Muang, Buriram, Thailand aut NATIONALLICENCE 700 1- Sanoamuang La-orsri Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University, 40002, Muang, Khon Kaen, Thailand aut NATIONALLICENCE 773 0- The Nucleus Springer-Verlag 53/3(2010-12-01), 103-107 0029-568X 53:3<103 2010 53 13237 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer