caa a22 4500 445301678 CHVBK 20180317142600.0 cr unu---uuuuu 170323e20100801xx s 000 0 eng 10.1007/s13237-010-0007-8 doi (NATIONALLICENCE)springer-10.1007/s13237-010-0007-8 Genome size, base composition and karyotype of Carica papaya L [Elektronische Daten] [Fernanda Araújo, Carlos Carvalho, Wellington Clarindo] Few data concerning Carica papaya DNA content and chromosomal characterization have been reported in specialized bibliography. Considering this fact, flow cytometry and cytogenetic methodologies were applied in order to improve the knowledge about the genome size, base composition and karyotype of C. papaya hermaphroditic plants. Flow cytometry results showed that hermaphroditic C. papaya genome size is relatively small (2C = 0.65pg) and the base composition (63.49% AT and 36.51% GC) is typical of core dicots. All mitotic cells showed 2n = 18 chromosomes, which were classified in metacentric or submetacentric. Despite small and homomorphic chromosomes, it has been made possible to resolve karyogram of hermaphroditic C. papaya. Through classical and banding techniques, the secondary constriction was observed in association to the chromosome 1 centromere. Here, we propose flow cytometry and cytogenetic protocols for studying the C. papaya genome. These methodologies may be useful as a basis for molecular cytogenetic techniques application. Archana Sharma Foundation of Calcutta, 2010 Carica papaya nationallicence Chromosomal characterization nationallicence DNA content nationallicence AT% nationallicence GC% nationallicence Araújo Fernanda Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Carvalho Carlos Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut Clarindo Wellington Laboratório de Microscopia, Departamento de Produção Vegetal, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, 29500-000, Alegre, ES, Brazil aut The Nucleus Springer-Verlag 53/1-2(2010-08-01), 25-31 0029-568X 53:1-2<25 2010 53 13237 https://doi.org/10.1007/s13237-010-0007-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13237-010-0007-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araújo Fernanda Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Carvalho Carlos Laboratório de Citogenética e Citometria, Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil aut NATIONALLICENCE 700 1- Clarindo Wellington Laboratório de Microscopia, Departamento de Produção Vegetal, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, 29500-000, Alegre, ES, Brazil aut NATIONALLICENCE 773 0- The Nucleus Springer-Verlag 53/1-2(2010-08-01), 25-31 0029-568X 53:1-2<25 2010 53 13237 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer