caa a22 4500 445301724 CHVBK 20180317142600.0 cr unu---uuuuu 170323e20100801xx s 000 0 eng 10.1007/s13237-010-0005-x doi (NATIONALLICENCE)springer-10.1007/s13237-010-0005-x Comparative evaluation of promutagens o -PDA, m -PDA and MH for genotoxic response in root cells of Allium cepa L [Elektronische Daten] [Manosij Ghosh, Jit Paul, Sonali Sinha, Anita Mukherjee] A wide number of chemicals from different sources are released into the environment, some of which upon metabolic activation are converted to mutagenic products. These chemicals might hence possess threat to public health. Genotoxic potential of three promutagens - o-phenylenediamine (o-PDA), m-phenylenediamine (m-PDA) and maleic hydrazide (MH) was evaluated using Allium cepa as the test system. Following treatment the genotoxic endpoints measured in the root tissue were mitotic index, cells with mitotic or chromosome aberration and micronuclei. DNA damage in root cells was evaluated by comet assay. EC50 determined on the basis of root growth for o-PDA, m-PDA, MH were between 7-8mg/l, 65-70mg/l and 9-10mg/l respectively. All the three agents induced genotoxicity significantly, that followed a dose response. While o-PDA and m-PDA did not induce DNA damage significantly, induction of DNA damage by MH was significant and dose dependent. The study identifies the genotoxic potential and re-establishes the fact that Allium cepa could ideally be used for evaluation of chromosome aberration and DNA damage. Archana Sharma Foundation of Calcutta, 2010 Chromosome aberrations nationallicence Comet assay nationallicence DNA damage nationallicence Micronuclei nationallicence Maleic hydrazide nationallicence MH : Maleic hydrazide nationallicence o -PDA : Ortho phenylenediamine nationallicence m -PDA : Meta phenylenediamine nationallicence OTM : Olive tail moment nationallicence MN : Micronuclei nationallicence MI : Mitotic index nationallicence Ghosh Manosij Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut Paul Jit Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut Sinha Sonali Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut Mukherjee Anita Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut The Nucleus Springer-Verlag 53/1-2(2010-08-01), 45-50 0029-568X 53:1-2<45 2010 53 13237 https://doi.org/10.1007/s13237-010-0005-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13237-010-0005-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghosh Manosij Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut NATIONALLICENCE 700 1- Paul Jit Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut NATIONALLICENCE 700 1- Sinha Sonali Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut NATIONALLICENCE 700 1- Mukherjee Anita Centre of Advanced Study, Cell and Chromosome Research, Department of Botany, University of Calcutta, 700 019, Kolkata, India aut NATIONALLICENCE 773 0- The Nucleus Springer-Verlag 53/1-2(2010-08-01), 45-50 0029-568X 53:1-2<45 2010 53 13237 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer