caa a22 4500 445303115 CHVBK 20180317142604.0 cr unu---uuuuu 170323e20100101xx s 000 0 eng 10.1007/s12274-010-1004-6 doi (NATIONALLICENCE)springer-10.1007/s12274-010-1004-6 Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments [Elektronische Daten] [Fuping Gao, Yuanyuan Cai, Jing Zhou, Xiaoxue Xie, Weiwei Ouyang, Yuhui Zhang, Xufei Wang, Xiaodong Zhang, Xiaowen Wang, Lingyun Zhao, Jintian Tang] Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe3O4 particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators. Tsinghua University Press and Springer Berlin Heidelberg, 2010 Magnetic nanoparticles nationallicence pullulan acetate nationallicence hyperthermia nationallicence cytotoxicity nationallicence cellular uptake nationallicence Gao Fuping Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Cai Yuanyuan Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Zhou Jing Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Xie Xiaoxue Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Ouyang Weiwei Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Zhang Yuhui Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Wang Xufei Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Zhang Xiaodong Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Wang Xiaowen Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Zhao Lingyun Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Tang Jintian Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut Nano Research Tsinghua Press 3/1(2010-01-01), 23-31 1998-0124 3:1<23 2010 3 12274 https://doi.org/10.1007/s12274-010-1004-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12274-010-1004-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Fuping Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Cai Yuanyuan Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhou Jing Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Xie Xiaoxue Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Ouyang Weiwei Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Yuhui Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wang Xufei Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Xiaodong Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wang Xiaowen Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhao Lingyun Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Tang Jintian Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Nano Research Tsinghua Press 3/1(2010-01-01), 23-31 1998-0124 3:1<23 2010 3 12274 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer