caa a22 4500 445303484 CHVBK 20180317142605.0 cr unu---uuuuu 170323e20100301xx s 000 0 eng 10.1007/s12274-010-1025-1 doi (NATIONALLICENCE)springer-10.1007/s12274-010-1025-1 Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube Raman tags in the near-infrared [Elektronische Daten] [Zhuang Liu, Scott Tabakman, Sarah Sherlock, Xiaolin Li, Zhuo Chen, Kaili Jiang, Shoushan Fan, Hongjie Dai] Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed Raman imaging of live cells has been carried out by highly specific staining of cells with a five-color mixture of SWNTs. Ex vivo multiplexed Raman imaging of tumor samples uncovers a surprising up-regulation of epidermal growth factor receptor (EGFR) on LS174T colon cancer cells from cell culture to in vivo tumor growth. This is the first time five-color multiplexed molecular imaging has been performed in the near-infrared (NIR) region under a single laser excitation. Near zero interfering background of imaging is achieved due to the sharp Raman peaks unique to nanotubes over the low, smooth autofluorescence background of biological species. Tsinghua University Press and Springer-Verlag Berlin Heidelberg, 2010 Carbon nanotubes nationallicence Raman scattering nationallicence biomedicine nationallicence multiplexed imaging nationallicence Liu Zhuang Functional Nano & Soft Materials Laboratory (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 215123, Suzhou, China aut Tabakman Scott Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut Sherlock Sarah Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut Li Xiaolin Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut Chen Zhuo Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut Jiang Kaili Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center, Tsinghua University, 100084, Beijing, China aut Fan Shoushan Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center, Tsinghua University, 100084, Beijing, China aut Dai Hongjie Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut Nano Research Tsinghua Press 3/3(2010-03-01), 222-233 1998-0124 3:3<222 2010 3 12274 https://doi.org/10.1007/s12274-010-1025-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12274-010-1025-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zhuang Functional Nano & Soft Materials Laboratory (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Tabakman Scott Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut NATIONALLICENCE 700 1- Sherlock Sarah Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut NATIONALLICENCE 700 1- Li Xiaolin Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut NATIONALLICENCE 700 1- Chen Zhuo Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut NATIONALLICENCE 700 1- Jiang Kaili Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Fan Shoushan Department of Physics and Tsinghua-Foxconn Nanotechnology Research Center, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Dai Hongjie Department of Chemistry, Stanford University, 94305, Stanford, CA, USA aut NATIONALLICENCE 773 0- Nano Research Tsinghua Press 3/3(2010-03-01), 222-233 1998-0124 3:3<222 2010 3 12274 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer