caa a22 4500 445303603 CHVBK 20180317142605.0 cr unu---uuuuu 170323e20101001xx s 000 0 eng 10.1007/s12274-010-0036-2 doi (NATIONALLICENCE)springer-10.1007/s12274-010-0036-2 Highly-sensitive multiplexed in vivo imaging using pegylated upconversion nanoparticles [Elektronische Daten] [Liang Cheng, Kai Yang, Shuai Zhang, Mingwang Shao, Shuittong Lee, Zhuang Liu] Lanthanide-based upconversion nanoparticles (UCNPs) have been widely explored in various fields, including optical imaging, in recent years. Although earlier work has shown that UCNPs with different lanthanide (Ln3+) dopants exhibit various colors, multicolor-especially in vivo multiplexed biomedical imaging-using UCNPs has rarely been reported. In this work, we synthesize a series of UCNPs with different emission colors and functionalize them with an amphiphilic polymer to confer water solubility. Multicolor in vivo upconversion luminescence (UCL) imaging is demonstrated by imaging subcutaneously injected UCNPs and applied in multiplexed in vivo lymph node mapping. We also use UCNPs for multicolor cancer cell labeling and realize in vivo cell tracking by UCL imaging. Moreover, for the first time we compare the in vivo imaging sensitivity of quantum dot (QD)-based fluorescence imaging and UCNP-based UCL imaging side by side, and find the in vivo detection limit of UCNPs to be at least one order of magnitude lower than that of QDs in our current non-optimized imaging system. Our data suggest that, by virtue of their unique optical properties, UCNPs have great potential for use in highly-sensitive multiplexed biomedical imaging. Tsinghua University Press and Springer-Verlag Berlin Heidelberg, 2010 Upconversion nanoparticles nationallicence multicolor imaging nationallicence lymphatic mapping nationallicence cell tracking nationallicence sensitive imaging nationallicence Cheng Liang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut Yang Kai Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut Zhang Shuai Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut Shao Mingwang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut Lee Shuittong Center of Super-Diamond and Advanced Films (COSDAF) and Department of Physics and Materials Science, City University of Hong Kong, Hong Kong, China aut Liu Zhuang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut Nano Research Tsinghua Press 3/10(2010-10-01), 722-732 1998-0124 3:10<722 2010 3 12274 https://doi.org/10.1007/s12274-010-0036-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12274-010-0036-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Liang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Yang Kai Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Shuai Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Shao Mingwang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut NATIONALLICENCE 700 1- Lee Shuittong Center of Super-Diamond and Advanced Films (COSDAF) and Department of Physics and Materials Science, City University of Hong Kong, Hong Kong, China aut NATIONALLICENCE 700 1- Liu Zhuang Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials, Soochow University, 215123, Suzhou, Jiangsu, China aut NATIONALLICENCE 773 0- Nano Research Tsinghua Press 3/10(2010-10-01), 722-732 1998-0124 3:10<722 2010 3 12274 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer