caa a22 4500 445303743 CHVBK 20180317142606.0 cr unu---uuuuu 170323e20100801xx s 000 0 eng 10.1007/s12274-010-0016-6 doi (NATIONALLICENCE)springer-10.1007/s12274-010-0016-6 One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection [Elektronische Daten] [Miao Cai, Feng Li, Yan Zhang, Qiangbin Wang] We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing. Tsinghua University Press and Springer-Verlag Berlin Heidelberg, 2010 deoxyribonucleic acid (DNA) detection nationallicence polymerase chain reaction (PCR) nationallicence Au nanoparticle nationallicence colorimetric nationallicence Cai Miao i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut Li Feng i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut Zhang Yan i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut Wang Qiangbin i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut Nano Research Tsinghua Press 3/8(2010-08-01), 557-563 1998-0124 3:8<557 2010 3 12274 https://doi.org/10.1007/s12274-010-0016-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12274-010-0016-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai Miao i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Li Feng i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Zhang Yan i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Wang Qiangbin i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 215123, Suzhou, China aut NATIONALLICENCE 773 0- Nano Research Tsinghua Press 3/8(2010-08-01), 557-563 1998-0124 3:8<557 2010 3 12274 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer