caa a22 4500 445304170 CHVBK 20180317142607.0 cr unu---uuuuu 170323e20100901xx s 000 0 eng 10.1007/s13246-010-0032-6 doi (NATIONALLICENCE)springer-10.1007/s13246-010-0032-6 Measurement of reoxygenation during fractionated radiotherapy in head and neck squamous cell carcinoma xenografts [Elektronische Daten] [Wendy Harriss, E. Bezak, E. Yeoh, M. Hermans] Hypoxic tissues lack adequate oxygenation and it has been long established that tumours commonly exhibit hypoxia and that hypoxia is a factor contributing towards resistance to radiotherapy. To develop computer models and make predictions about the affects of tumour hypoxia on treatment outcome, quantitative tumour oxygenation and reoxygenation data from in vivo systems is required. The aim of this study was to investigate the timing and degree of reoxygenation during radiotherapy in a human head and neck squamous cell carcinoma xenograft mouse model (FaDu). Mice were immobilised using a novel restraining system and exposed unanaesthetised in 3 or 5Gy fractions, up to a maximum of 40Gy. Partial pressures of oxygen (pO2) measurements were recorded at six time points throughout the 2 week course of radiotherapy, using a fibre optic system. Tumours receiving 0-30Gy did not exhibit an increase in pO2. However, the mean pO2 after 2 weeks of accelerated fractionated radiotherapy (40Gy) was significantly increased (P<0.01) compared to the mean pO2 of tumours not receiving the full schedule (0-30Gy). These results lead to the conclusion of an average reoxygenation onset time of 2 weeks in this group of xenografts. A relatively large range of pO2 values measured at each dose point in the study indicate a large inter-tumour variation in oxygenation among the tumours. Data from this experimental work will be used to define the range of reoxygenation onset times implemented in a Monte Carlo computer model, simulating hypoxic head and neck cancer growth and radiotherapy. Australasian College of Physical Scientists and Engineers in Medicine, 2010 Hypoxia nationallicence Radiotherapy nationallicence Reoxygenation nationallicence Monte Carlo nationallicence FaDu nationallicence Harriss Wendy School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut Bezak E. School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut Yeoh E. School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut Hermans M. School of Medical Physics and Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands aut Australasian Physical & Engineering Sciences in Medicine Springer Netherlands 33/3(2010-09-01), 251-263 0158-9938 33:3<251 2010 33 13246 https://doi.org/10.1007/s13246-010-0032-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s13246-010-0032-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harriss Wendy School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut NATIONALLICENCE 700 1- Bezak E. School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut NATIONALLICENCE 700 1- Yeoh E. School of Chemistry and Physics, University of Adelaide, Adelaide, Australia aut NATIONALLICENCE 700 1- Hermans M. School of Medical Physics and Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands aut NATIONALLICENCE 773 0- Australasian Physical & Engineering Sciences in Medicine Springer Netherlands 33/3(2010-09-01), 251-263 0158-9938 33:3<251 2010 33 13246 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer