caa a22 4500 445306734 CHVBK 20180627064139.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00404-010-1520-3 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1520-3 Dose-response effect of interleukin (IL)-1β, tumour necrosis factor (TNF)-α, and interferon-γ on the in vitro production of epithelial neutrophil activating peptide-78 (ENA-78), IL-8, and IL-6 by human endometrial stromal cells [Elektronische Daten] [Nick Bersinger, Andreas Günthert, Brett McKinnon, Silke Johann, Michael Mueller] Purpose: The production of epithelial neutrophil activating peptide-78 (NA-78) and the interleukins IL-8 and IL-6 by endometrial stromal cells is stimulated by pro-inflammatory interleukin-1 (IL-1) and tumour necrosis factor-α (TNF-α). IL-8 is suggested to play a role in the pathogenesis of endometriosis, and in these women the peritoneal fluid concentrations of ENA-78 and IL-8 are increased. TNF-α has been tested together with interferon-γ because of their cooperative stimulation of IL-6. The release of IL-8, however, is inhibited with increasing interferon levels. The aim of the study was the analysis of the production of ENA-78, IL-6 and IL-8 by cultured human endometrial stromal cells in the presence of varying concentrations of IL-1β, TNF-α, and interferon-γ. Methods: Eutopic endometrial tissue was obtained from seven cycling, endometriosis-free women undergoing laparoscopy for reasons of infertility or pain. The release of ENA-78, IL-8 and IL-6 by the isolated and monolayer cultured stromal cell fraction in the presence of IL-1β (0.08 to 50ng/mL), TNF-α, and interferon-γ (both 20 to 500ng/mL) was determined. Results: IL-1β stimulated the production of IL-8, IL-6, and ENA-78 dose dependently from 0.08 to 2.0ng/mL (ENA-78) or to 10ng/mL (IL-8, IL-6); at 50ng/mL a decrease in release was observed for IL-8 and IL-6. TNF-α stimulation yielded a plateau between 20 and 100ng/mL. Interferon-γ stimulated IL-6 and inhibited IL-8 production above 20ng/mL. ENA-78 release was largely unaffected by interferon-γ. Conclusions: IL-1β and TNF-α stimulate stromal cytokine production cumulatively with different dose-response curves. The presence of interferon-γ has opposite effects on IL-8 and IL-6. TNF-α and interferon-γ should be investigated separately in future in vitro studies with endometrial cells and explants. Springer-Verlag, 2010 Endometrium nationallicence Cytokine stimulation nationallicence Epithelial neutrophil activating peptide-78 nationallicence Interleukins nationallicence Interferon nationallicence Bersinger Nick Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut Günthert Andreas Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut McKinnon Brett Department of Clinical Research, University of Berne, Berne, Switzerland aut Johann Silke Hôpital du jura bernois, St-Imier, Switzerland aut Mueller Michael Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut Archives of Gynecology and Obstetrics Springer-Verlag 283/6(2011-06-01), 1291-1296 0932-0067 283:6<1291 2011 283 404 https://doi.org/10.1007/s00404-010-1520-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1520-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bersinger Nick Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut NATIONALLICENCE 700 1- Günthert Andreas Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut NATIONALLICENCE 700 1- McKinnon Brett Department of Clinical Research, University of Berne, Berne, Switzerland aut NATIONALLICENCE 700 1- Johann Silke Hôpital du jura bernois, St-Imier, Switzerland aut NATIONALLICENCE 700 1- Mueller Michael Department of Obstetrics and Gynaecology, University of Berne, Berne, Switzerland aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/6(2011-06-01), 1291-1296 0932-0067 283:6<1291 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 445306734 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer