caa a22 4500 445306904 CHVBK 20180317142616.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00404-010-1583-1 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1583-1 Effects of peritoneal fluid from endometriosis patients on the release of monocyte-specific chemokines by leukocytes [Elektronische Daten] [Yong-Jin Na, Dong-Hyung Lee, Seung-Chul Kim, Jong-Kil Joo, Ji-Won Wang, Jun-O Jin, Jong-Young Kwak, Kyu-Sup Lee] Purpose: Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted (RANTES), and macrophage inflammatory protein-1α (MIP-1α) by neutrophils, monocytes, and T cells. Moreover, we evaluated the correlation between the levels of chemokines in ePF and their release by these cells. Methods: Cells were obtained from healthy young volunteers and cultured with ePF (n=12) or cPF (n=8). The chemokine levels in the ePF and the supernatants of cultured cells with ePF were then measured by ELISA. Results: There was a positive correlation between the levels of MCP-1 and MIP-1α in ePF. The addition of ePF to the cell cultures failed to increase the release of MCP-1, RANTES, and MIP-1α when compared to cPF, but the levels of RANTES in ePF were positively correlated with the release of RANTES by ePF-treated monocytes and T cells. Moreover, there was a positive correlation between the levels of RANTES and MIP-1α released by neutrophils and between the levels of MCP-1 and MIP-1α released by T cells. Finally, the levels of RANTES released by monocyte-derived macrophages and monocytes cultured with ePF were positively correlated. Conclusions: These findings suggest that monocytes, neutrophils, and T cells release differential levels of MCP-1, RANTES, and MIP-1α in response to stimulation with ePF. Springer-Verlag, 2010 Endometriosis nationallicence CC chemokines nationallicence Monocytes nationallicence Neutrophils nationallicence T cells nationallicence Na Yong-Jin Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut Lee Dong-Hyung Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut Kim Seung-Chul Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut Joo Jong-Kil Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut Wang Ji-Won Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut Jin Jun-O Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut Kwak Jong-Young Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut Lee Kyu-Sup Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut Archives of Gynecology and Obstetrics Springer-Verlag 283/6(2011-06-01), 1333-1341 0932-0067 283:6<1333 2011 283 404 https://doi.org/10.1007/s00404-010-1583-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1583-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Na Yong-Jin Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut NATIONALLICENCE 700 1- Lee Dong-Hyung Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut NATIONALLICENCE 700 1- Kim Seung-Chul Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut NATIONALLICENCE 700 1- Joo Jong-Kil Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut NATIONALLICENCE 700 1- Wang Ji-Won Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut NATIONALLICENCE 700 1- Jin Jun-O Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut NATIONALLICENCE 700 1- Kwak Jong-Young Medical Research Center for Cancer Molecular Therapy and Department of Biochemistry, School of Medicine, Dong-A University, 602-714, Busan, Korea aut NATIONALLICENCE 700 1- Lee Kyu-Sup Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, 602-739, Busan, Korea aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/6(2011-06-01), 1333-1341 0932-0067 283:6<1333 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer