caa a22 4500 445307196 CHVBK 20180317142616.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00404-010-1427-z doi (NATIONALLICENCE)springer-10.1007/s00404-010-1427-z Microbiological characteristics and inflammatory cytokines associated with preterm labor [Elektronische Daten] [Michelle Discacciati, Jose Simoes, Marcia Silva, Camila Marconi, Eliane Brolazo, Maria Costa, Jose Cecatti] Purpose: To evaluate vaginal microflora and interleukin-1β (IL-β), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) concentrations in the cervicovaginal fluid of a group of pregnant women in preterm labor when compared with a group of full-term pregnant women not yet in labor. Method: Case-control study performed in a University tertiary referral maternity in Campinas, Brazil with 45 pregnant women in preterm labor and 45 full-term pregnant women not in labor. All patients underwent speculum examination for the collection of cervicovaginal fluid. Bacterial vaginosis (BV) was diagnosed according to the criteria of Amsel and Nugent. Culture was performed for group B streptococcus (GBS) and lactobacilli, and hybrid capture assay for screening for chlamydial and gonococcal infection. Cytokine concentrations were measured using ELISA technique. Statistical analysis was performed using χ2, Fisher's exact, and crude and adjusted odds ratios. Significance level was defined at 5%. The main outcome measures were cervicovaginal cytokines in preterm labor. Results: IL-6 and IL-8 were significantly associated with preterm labor. The changes in vaginal microflora, as well as BV and GBS, were more frequent in women in preterm labor, although BV and GBS showed no statistical significance. The presence of Candida sp., absence of lactobacilli, positive screening for chlamydial and gonococcal infection and the presence of IL-1β and TNF-α were not associated with preterm labor. Conclusions: IL-6 and IL-8 and the presence of any type of vaginal infection were the factors that were significantly associated with preterm labor. Springer-Verlag, 2010 Preterm labor nationallicence Cytokines nationallicence Interleukins nationallicence Bacterial vaginosis nationallicence BMI : Body mass index nationallicence BV : Bacterial vaginosis nationallicence CI : Confidence interval nationallicence CT : Chlamydia trachomatis nationallicence GBS : Group B Streptococcus nationallicence IL : Interleukin nationallicence LPS : Lipopolysaccharide nationallicence NG : Neisseria gonorrhea nationallicence OR : Odds ratio nationallicence PBS : Phosphate buffered saline nationallicence RLU : Relative light unit nationallicence TNF-α : Tumor necrosis factor-alpha nationallicence UNICAMP : University of Campinas nationallicence UTI : Urinary tract infection nationallicence Discacciati Michelle Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut Simoes Jose aut Silva Marcia Department of Pathology, Universidade Estadual Paulista "Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil aut Marconi Camila Department of Pathology, Universidade Estadual Paulista "Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil aut Brolazo Eliane Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut Costa Maria Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut Cecatti Jose Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut Archives of Gynecology and Obstetrics Springer-Verlag 283/3(2011-03-01), 501-508 0932-0067 283:3<501 2011 283 404 https://doi.org/10.1007/s00404-010-1427-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1427-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Discacciati Michelle Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Simoes Jose aut NATIONALLICENCE 700 1- Silva Marcia Department of Pathology, Universidade Estadual Paulista "Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil aut NATIONALLICENCE 700 1- Marconi Camila Department of Pathology, Universidade Estadual Paulista "Júlio de Mesquita Filho” (UNESP), Botucatu, Brazil aut NATIONALLICENCE 700 1- Brolazo Eliane Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Costa Maria Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Cecatti Jose Department of Obstetrics and Gynecology, School of Medical Sciences, Universidade Estadual De Campinas, Campinas, SP, Brazil aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/3(2011-03-01), 501-508 0932-0067 283:3<501 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer