caa a22 4500 44530796X CHVBK 20180317142619.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00404-010-1511-4 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1511-4 Molecular characterization of early adenocarcinoma of the uterine cervix by oligonucleotide microarray [Elektronische Daten] [Ling-Min Liao, Min Zheng, Long Huang, Hui Ding, Li He, Ying Zhang, Hui-Yun Wang] Purpose: In an attempt to understand the molecular characterization for the early adenocarcinoma of the uterine cervix, an analysis of gene expression profiles obtained from early adenocarcinoma of the uterine cervix was performed to find those genes most aberrantly expressed. Methods and materials: Total RNA was prepared from 32 samples of early adenocarcinoma of the uterine cervix and 32 paired normal cervix tissues, and hybridized to cancer-associated oligonucleotide microarrays with probe sets complementary to about 1,426 transcripts. Results: Supervised analysis of gene expression data identified 13 genes that exhibited >2-fold upregulation and 27 genes >2-fold downregulation, respectively, in early adenocarcinoma of the uterine cervix compared to normal cervix. Unsupervised hierarchical clustering of the expression data readily distinguished early adenocarcinoma of the uterine cervix from normal cervix. Two genes (karyopherin alpha 2 and proliferating cell nuclear antigen) were selected randomly for real-time reverse transcription polymerase chain reaction analysis. Both genes were expressed significantly higher in early adenocarcinoma of the uterine cervix than in normal cervix, with p=0.0003 and <0.0001, respectively. These results were compatible with the microarray data. Conclusions: This study has revealed several genes that may be highly attractive candidate molecular markers/targets for early adenocarcinoma of the uterine cervix. Springer-Verlag, 2010 Adenocarcinoma nationallicence Uterine cervix nationallicence Gene expression nationallicence Oligonucleotide microarray nationallicence Liao Ling-Min Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, 515041, Shantou, China aut Zheng Min State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut Huang Long State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut Ding Hui State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut He Li State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut Zhang Ying Department of Sample Bank, Cancer Center, Sun Yat-sen University, 510060, Guangzhou, China aut Wang Hui-Yun State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut Archives of Gynecology and Obstetrics Springer-Verlag 283/4(2011-04-01), 861-869 0932-0067 283:4<861 2011 283 404 https://doi.org/10.1007/s00404-010-1511-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1511-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Ling-Min Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, 515041, Shantou, China aut NATIONALLICENCE 700 1- Zheng Min State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut NATIONALLICENCE 700 1- Huang Long State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut NATIONALLICENCE 700 1- Ding Hui State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut NATIONALLICENCE 700 1- He Li State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Ying Department of Sample Bank, Cancer Center, Sun Yat-sen University, 510060, Guangzhou, China aut NATIONALLICENCE 700 1- Wang Hui-Yun State Key Laboratory of Oncology in Southern China, 651 Dongfeng Road East, 510060, Guangzhou, China aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/4(2011-04-01), 861-869 0932-0067 283:4<861 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer