caa a22 4500 44530832X CHVBK 20180317142620.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00404-010-1546-6 doi (NATIONALLICENCE)springer-10.1007/s00404-010-1546-6 Expression and methylation status of the Syk gene in cervical carcinoma [Elektronische Daten] [Shuping Zhao, Guixia Sun, Parks Tony, Dehua Ma, Chengquan Zhao] Objective: Spleen tyrosine kinase (Syk), a non-receptor protein tyrosine kinase, has recently been recognized as a new candidate tumor suppressor. Decrease or loss of Syk expression has been associated with a malignant phenotype and poor prognosis in a variety of cancers. This study aimed to determine the precise role of Syk in cervical cancer. Methods: Methylation-specific PCR (MSP) and RT-PCR were utilized to analyze the methylation status and Syk mRNA expression in tissue samples from 20 normal controls, 50 CIN patients and 60 cervical cancer patients. Results: Syk expression was detected in all 20 normal cervical tissues, as well as in all 18 CIN1 samples. Syk expression was found in 18 of 32 (56%) of CIN2/3 samples. Conclusion: The results indicate a potential link between the loss of Syk expression and cervical carcinogenesis. Springer-Verlag, 2010 Cervical cancer nationallicence Spleen tyrosine kinase nationallicence mRNA expression nationallicence Gene methylation nationallicence Cervical neoplasm nationallicence Gene nationallicence Suppressor nationallicence DNA methylation nationallicence Zhao Shuping Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut Sun Guixia Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut Tony Parks Department of Pathology, Magee Womens Hospital, University of Pittsburgh Medical Center, 300 Halket Street, 15213, Pittsburgh, PA, USA aut Ma Dehua Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut Zhao Chengquan Department of Pathology, Magee Womens Hospital, University of Pittsburgh Medical Center, 300 Halket Street, 15213, Pittsburgh, PA, USA aut Archives of Gynecology and Obstetrics Springer-Verlag 283/5(2011-05-01), 1113-1119 0932-0067 283:5<1113 2011 283 404 https://doi.org/10.1007/s00404-010-1546-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1546-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Shuping Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Sun Guixia Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Tony Parks Department of Pathology, Magee Womens Hospital, University of Pittsburgh Medical Center, 300 Halket Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Ma Dehua Department of Gynecology, Affiliated Hospital of Medical College of Qingdao University, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Zhao Chengquan Department of Pathology, Magee Womens Hospital, University of Pittsburgh Medical Center, 300 Halket Street, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/5(2011-05-01), 1113-1119 0932-0067 283:5<1113 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer