caa a22 4500 445308931 CHVBK 20180317142622.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00404-009-1348-x doi (NATIONALLICENCE)springer-10.1007/s00404-009-1348-x Genetic variation in the CYP17 gene and recurrent spontaneous abortions [Elektronische Daten] [Iakovos Litridis, Neoptolemos Kapnoulas, Theona Natisvili, Konstantinos Agiannitopoulos, Ourania Peraki, Panagiotis Ntostis, Konstantinos Pantos, Klea Lamnissou] Purpose: The CYP17 gene encodes the enzyme cytochrome P450c17α, which functions at key steps in the synthesis process of human sex steroid hormones. A T/C polymorphism in the 5′ promoter region of the CYP17 gene has been described previously. Serum levels of androgens and estrogens have been shown to be elevated in individuals who carry the C substitution (Α2 allele). We hypothesized that variability in genes that control the sex hormone (estrogens, testosterone) biosynthesis might affect the pregnancy outcome. In the present study, we investigated the possible association between the T/C polymorphism of the promoter of CYP17 gene and the risk of recurrent spontaneous abortions in the Greek population. Methods: In the prospective case-control study, 148 patients and 134 healthy controls were studied. Women who had at least three unexplained spontaneous abortions before 20weeks of gestation were included in the patient group. The PCR-RFLP method was used to genotype the subjects. Results: The frequencies of A1A1, A1A2, A2A2 genotypes were 0.34, 0.52, 0.14, respectively, in the patient group and 0.32, 0.47, 0.21, respectively, in the control group. The allele frequencies were 0.595 and 0.405 for A1 and A2, in the patient group and 0.555 and 0.445 for A1 and A2, respectively, in the control group. The data between the two groups were analyzed by χ2 test. Our results showed that there are no significant differences in genotype (P=0.3883) or in allele frequencies (P=0.3800) between the patient and the control group. Conclusion: The T/C promoter polymorphism of the CYP17 gene is not associated with the risk for recurrent spontaneous abortions in our Caucasian population. Springer-Verlag, 2010 Recurrent spontaneous abortions nationallicence Genetic polymorphisms nationallicence CYP17 gene nationallicence Risk factor nationallicence Litridis Iakovos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Kapnoulas Neoptolemos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Natisvili Theona Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Agiannitopoulos Konstantinos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Peraki Ourania Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Ntostis Panagiotis Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Pantos Konstantinos "Genesis” Athens Clinic, Center for Human Reproduction, Athens, Greece aut Lamnissou Klea Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut Archives of Gynecology and Obstetrics Springer-Verlag 283/2(2011-02-01), 289-293 0932-0067 283:2<289 2011 283 404 https://doi.org/10.1007/s00404-009-1348-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-009-1348-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Litridis Iakovos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Kapnoulas Neoptolemos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Natisvili Theona Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Agiannitopoulos Konstantinos Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Peraki Ourania Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Ntostis Panagiotis Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 700 1- Pantos Konstantinos "Genesis” Athens Clinic, Center for Human Reproduction, Athens, Greece aut NATIONALLICENCE 700 1- Lamnissou Klea Department of Genetics and Biotechnology, Faculty of Biology, University of Athens, Athens, Greece aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 283/2(2011-02-01), 289-293 0932-0067 283:2<289 2011 283 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer