caa a22 4500 445309148 CHVBK 20180317142623.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00404-010-1586-y doi (NATIONALLICENCE)springer-10.1007/s00404-010-1586-y The effect of cigarette smoking on cell proliferation in normal uterine cervix epithelium [Elektronische Daten] [Adriana Campaner, Aline Eras, Camila Paganini, Maria Galvão] Background: The aim of this study was to evaluate the effect of smoking on cell proliferation in normal cervical epithelium. Methods: A total of 45 women undergoing hysterectomies due to uterine leiomyomata were investigated. Thirty were nonsmokers and 15 were current smokers. A segment of tissue was taken from cervix after removal of the uterus, and the identification of cell proliferation was performed by immunohistochemical analysis employing Ki-67 staining. The number of positive cells colored by Ki-67 staining was finally expressed as number of cells per square millimeter (mm2) of epithelium. Results: The comparison of the number of proliferating cells measured by ki-67 staining among smoking and nonsmoking women did not show significant difference. Among smoking women, there was no significant difference related to the number of cigarettes smoked per day or time of consumption and epithelial cell proliferation. However, the total amount of cigarettes smoked throughout presented significant association with Ki-67 staining (p<0.001); the number of proliferating cells per mm2 increased proportionally to the increase in consumption of cigarettes. Conclusions: The results indicated that there was no significant difference between cell proliferation in smokers and nonsmokers; however, it was shown that in the smokers' group, amount of cigarettes smoked during throughout lifetime can increase cell proliferation in normal cervical epithelium. Springer-Verlag, 2010 Cell proliferation nationallicence Cervix nationallicence Smoking nationallicence Carcinogenesis nationallicence Campaner Adriana Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut Eras Aline Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut Paganini Camila Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut Galvão Maria Department of Pathology, Medical Science College of Santa Casa of São Paulo, São Paulo, Brazil aut Archives of Gynecology and Obstetrics Springer-Verlag 284/1(2011-07-01), 119-123 0932-0067 284:1<119 2011 284 404 https://doi.org/10.1007/s00404-010-1586-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-010-1586-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Campaner Adriana Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Eras Aline Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Paganini Camila Department of Obstetrics and Gynecology, Medical Science College of Santa Casa of São Paulo, Av. Rebouças, 1511 ap. 142, Jd. América, 05401-200, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Galvão Maria Department of Pathology, Medical Science College of Santa Casa of São Paulo, São Paulo, Brazil aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/1(2011-07-01), 119-123 0932-0067 284:1<119 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer