caa a22 4500 445309601 CHVBK 20180317142624.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00404-011-1891-0 doi (NATIONALLICENCE)springer-10.1007/s00404-011-1891-0 Biologically inhibitory effects of VEGF siRNA on endometrial carcinoma cells [Elektronische Daten] [Shuping Zhao, Dehua Ma, Hongying Dai, Fang Yuan, Zenyan Wang] Objective: To study the biological effects of small inhibitory RNA (siRNA) on endometrial carcinoma cells through disrupting the mRNA of Vascular endothelial growth factor (VEGF), and its inhibitory functions on tumor cells' proliferation. Methods: The sequence-specific siRNA of VEGF165 and the mock siRNA were designed, they were transfected into the endometrial carcinoma cell line, Ishikawa cells. Total cell RNAs were extracted from the transfected cells. The mRNA levels of VEGF were then analyzed utilizing real-time fluorescence quantitative RT-PCR (FQ-PCR) post-transfection at 12, 24, 48, 72, and 168h, respectively. Simultaneously its protein levels were also determined by western blot. Cell proliferations were monitored by MTT assay and cytopathological effects (CPE) were determined by Multispect Imaging System. Result: The mRNA and protein levels of VEGF declined post-transfection at 12, 24, 48, 72h, respectively, with a maximum decrease post-transfection at 48h. The proliferation of Ishikawa cells was significantly inhibited in a similar manner during this time window. All the data were repeated at least three independent times and similar results were achieved. Conclusion: The small siRNA of VEGF165 can effectively down-regulate its target mRNA and protein levels; consequently proliferation of Ishikawa cells was inhibited. The data strongly imply that VEGF may be involved in the genesis of endometrial carcinoma, which provides a new pathway to treat human endometrial caners. Springer-Verlag, 2011 RNA interference (RNAi) nationallicence Vascular endothelial growth factor (VEGF) nationallicence Real-time fluorescence quantitative RT-PCR (FQ-PCR) nationallicence Endometrial neoplasm nationallicence Zhao Shuping Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut Ma Dehua Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut Dai Hongying Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut Yuan Fang Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut Wang Zenyan Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1533-1541 0932-0067 284:6<1533 2011 284 404 https://doi.org/10.1007/s00404-011-1891-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-011-1891-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Shuping Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Ma Dehua Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Dai Hongying Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Fang Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zenyan Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University School of Medicine, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1533-1541 0932-0067 284:6<1533 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer