caa a22 4500 445309644 CHVBK 20180317142625.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00404-011-1990-y doi (NATIONALLICENCE)springer-10.1007/s00404-011-1990-y Cytogenetic, Y chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions [Elektronische Daten] [S. Venkatesh, J. Thilagavathi, K. Kumar, D. Deka, P. Talwar, Rima Dada] Purpose: Etiology in majority of couples experiencing recurrent spontaneous abortions (RSA) is still unknown. The aim of the study was to find the role of cytogenetic abnormalities, Y chromosome microdeletion, oxidative stress (OS) and sperm DNA fragmentation in male partners of couples experiencing RSA. Methods: Forty-eight couples with history of RSA and 20 fertile controls were included in the study. The study subjects were divided into male partners of RSA couples with abnormal sperm parameters (SA) (N=16), male partners of RSA couples with normal sperm parameters (NS) (N=32) and age-matched fertile controls with normal sperm parameters (FC) (N=20). Results: One of 48 men (2%) showed 46, XY (1qh-) chromosomal complement. None of the cases including FC showed deletion in any of the 3 AZF loci on Y chromosome long arm. Sperm count was found be significantly lower in SA cases as compared to group NS cases (P<0.0001) and FC (P<0.005). Sperm forward motility was found to be significantly (P<0.05) lower in SA cases as compared to NS and FC. Male partners of RSA couples with abnormal sperm parameters had higher reactive oxygen species (ROS) levels (P<0.005) and sperm DNA damage (P<0.0001), however, in male partners of RSA couples with normal sperm parameters had only increased (P<0.0001) sperm DNA damage. Conclusion: Other than chromosomal anomalies, sperm DNA fragmentation and seminal OS may be the underlying pathology in RSA, thus screening for seminal ROS levels and DNA fragmentation has diagnostic and prognostic capabilities. Springer-Verlag, 2011 Recurrent spontaneous abortions nationallicence Sperm DNA fragmentation nationallicence Oxidative stress nationallicence Reactive oxygen species nationallicence Semen parameters nationallicence Venkatesh S. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut Thilagavathi J. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut Kumar K. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut Deka D. Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi, India aut Talwar P. ART Centre, Army Hospital, New Delhi, India aut Dada Rima Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1577-1584 0932-0067 284:6<1577 2011 284 404 https://doi.org/10.1007/s00404-011-1990-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-011-1990-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venkatesh S. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut NATIONALLICENCE 700 1- Thilagavathi J. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut NATIONALLICENCE 700 1- Kumar K. Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut NATIONALLICENCE 700 1- Deka D. Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 700 1- Talwar P. ART Centre, Army Hospital, New Delhi, India aut NATIONALLICENCE 700 1- Dada Rima Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science (AIIMS), 110029, New Delhi, India aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1577-1584 0932-0067 284:6<1577 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer