caa a22 4500 445309741 CHVBK 20180317142625.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00404-011-1875-0 doi (NATIONALLICENCE)springer-10.1007/s00404-011-1875-0 Bridge-1 is expressed in human breast carcinomas: silencing of Bridge-1 decreases Smad2, Smad3 and Smad4 expression in MCF-7 cells, a human breast cancer cell line [Elektronische Daten] [Constanze Banz-Jansen, Birte Münchow, Klaus Diedrich, Dominique Finas] Purpose: The aim of the study was to investigate the expression of Bridge-1 in human breast carcinomas, and to determine the in vitro regulation of Bridge-1 by activin A and the influence of Bridge-1 on activin A signaling in the human breast cancer cell line MCF-7. Method: Bridge-1 expression in human breast carcinomas was shown after staining paraffin slides with a specific antibody against Bridge-1. To gain insight into Bridge-1 function, immortalized, human breast cancer cells (MCF-7 cell line) were stimulated with activin A and the expression of Bridge-1 was analyzed by real-time PCR and Western blot. Next, Bridge-1 was downregulated via siRNA treatment in MCF-7 cells and the expression of Bridge-1, Smad2, 3 and 4 was investigated by real-time PCR and Western blot. Results: Human breast carcinoma cells showed nuclear and cytoplasmic localization of Bridge-1. Activin A stimulation of the immortalized human breast adenocarcinoma cell line MCF-7 showed an increase in Bridge-1 expression by real-time PCR and Western blot. Downregulation of Bridge-1 by Bridge-1-siRNA resulted in a decreased expression of Smad2, 3 and 4 of up to 50% compared to the treatment with non-targeting siRNA. Conclusions: This study is the first to demonstrate the expression of Bridge-1 in human breast carcinomas. Bridge-1 expression is increased by activin A stimulation and itself seems to influence activin A signaling by affecting the expression of Smad2, 3 and 4. Springer-Verlag, 2011 Bridge-1 nationallicence Human breast carcinomas nationallicence Activin A nationallicence Smads nationallicence MCF-7 nationallicence Banz-Jansen Constanze Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut Münchow Birte Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut Diedrich Klaus Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut Finas Dominique Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1543-1549 0932-0067 284:6<1543 2011 284 404 https://doi.org/10.1007/s00404-011-1875-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-011-1875-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banz-Jansen Constanze Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Münchow Birte Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Diedrich Klaus Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut NATIONALLICENCE 700 1- Finas Dominique Department for Gynecology and Obstetrics, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538, Lübeck, Germany aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1543-1549 0932-0067 284:6<1543 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer