caa a22 4500 445309784 CHVBK 20180317142625.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00404-011-2002-y doi (NATIONALLICENCE)springer-10.1007/s00404-011-2002-y Expression and localization of CXCL16 and CXCR6 in ovarian endometriotic tissues [Elektronische Daten] [Shuichi Manabe, Akira Iwase, Maki Goto, Hiroharu Kobayashi, Sachiko Takikawa, Yoshinari Nagatomo, Tatsuo Nakahara, Bayasula,, Tomoko Nakamura, Wakana Hirokawa, Fumitaka Kikkawa] Purpose: Inflammatory mediators, including chemokines, may play crucial roles in the development of endometriosis. Therefore, we investigated the expression and localization of CXCL16 and its receptor, CXCR6, in ovarian endometriotic tissues. We also examined whether CXCL16 induces IL-8 production in endometriotic stromal cells. Methods: We performed immunohistochemical and Western blotting analyses of in vivo and in vitro samples. IL-8 production was assayed using an ELISA. Results: Both CXCL16 and CXCR6 were expressed by endometriotic epithelial cells and stromal cells, but not normal ovarian stroma. A Western blotting analysis using primary cultured endometriotic stromal cells showed a constant expression of CXCL16 and CXCR6 in the proliferative phase, secretory phase and during gonadotropin-releasing hormone agonist therapy. CXCL16 induced IL-8 production in several endometriotic stromal cells in vitro. Conclusions: CXCL16 and CXCR6 might be involved in the pathophysiology of endometriosis through regulation of the inflammatory response. Springer-Verlag, 2011 CXCL16 nationallicence CXCR6 nationallicence Endometriosis nationallicence IL-8 nationallicence Manabe Shuichi Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Iwase Akira Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Goto Maki Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Kobayashi Hiroharu Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Takikawa Sachiko Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Nagatomo Yoshinari Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Nakahara Tatsuo Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Bayasula, Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Nakamura Tomoko Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Hirokawa Wakana Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Kikkawa Fumitaka Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1567-1572 0932-0067 284:6<1567 2011 284 404 https://doi.org/10.1007/s00404-011-2002-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-011-2002-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manabe Shuichi Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Iwase Akira Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Goto Maki Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Kobayashi Hiroharu Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Takikawa Sachiko Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Nagatomo Yoshinari Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakahara Tatsuo Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Bayasula Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Nakamura Tomoko Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Hirokawa Wakana Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 700 1- Kikkawa Fumitaka Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, 466-8550, Nagoya, Japan aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/6(2011-12-01), 1567-1572 0932-0067 284:6<1567 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer