caa a22 4500 445309881 CHVBK 20180317142626.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00404-011-1933-7 doi (NATIONALLICENCE)springer-10.1007/s00404-011-1933-7 Antioxidant prophylaxis for cellular injury in ovarian surface epithelium resulting from CO2 pneumoperitoneum in a laparoscopic rat model [Elektronische Daten] [Sule Kıray, Gogsen Onalan, Gulten Karabay, Hulusi Zeyneloglu, Esra Kuscu] Objective: Selective cytoprotective functions of vitamin E, N-acetyl-l-cysteine, and amifostine have been used as a preventer of ischemia injury by expelling the free oxygen radicals leading to stabilization of the cellular membranes. The purpose of this experimental study was to investigate the oxidative stress related to cellular injury in ovarian surface epithelium and the effect of prophylaxis with an anti-oxidant using laparoscopic rat model. Design: Laparoscopic rat model. Materials and methods: Randomly allocated 40 Wistar Albino female rats have been used for the pneumoperitoneum model which was constituted to fix the intraabdominal pressure on 5mmHg for 60min. The antioxidants, vitamin E and NAC were given to rats 3days before the operation and were applied for 30days; amifostine was applied 30min before the operation until after for 7days. After abdominal desufflation, over biopsies were made on the 13th min, 24th h, and 7th and 30th days. By using of transmission electron microscopy, the damage on cells and organels were assessed and graded. Results: In ovarian surface epithelium, the apical surface specializations were affected in all groups except Vit E group:The microvilli were irregular and coarse and had disappeared in some places. Some cells were separated from the epithelium. In addition, mitochondria degeneration was observed in all group except Vit E. Conclusions: In the early period of laparoscopy, reversible cellular damage occurs and this damage can be prevented by vitamin E. Springer-Verlag, 2011 Laparoscopy nationallicence Ovarian surface epithelium nationallicence CO2 pneumoperitoneum nationallicence Rat nationallicence Antioxidant nationallicence Vitamin E nationallicence Amifostine nationallicence Kıray Sule Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut Onalan Gogsen Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut Karabay Gulten Department of Histology, Baskent University, Ankara, Turkey aut Zeyneloglu Hulusi Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut Kuscu Esra Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut Archives of Gynecology and Obstetrics Springer-Verlag 284/3(2011-09-01), 765-772 0932-0067 284:3<765 2011 284 404 https://doi.org/10.1007/s00404-011-1933-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00404-011-1933-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kıray Sule Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut NATIONALLICENCE 700 1- Onalan Gogsen Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut NATIONALLICENCE 700 1- Karabay Gulten Department of Histology, Baskent University, Ankara, Turkey aut NATIONALLICENCE 700 1- Zeyneloglu Hulusi Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut NATIONALLICENCE 700 1- Kuscu Esra Department of Obstetrics and Gynecology, Baskent University School of Medicine, Kubilay Sok no: 36 Maltepe, 06570, Ankara, Turkey aut NATIONALLICENCE 773 0- Archives of Gynecology and Obstetrics Springer-Verlag 284/3(2011-09-01), 765-772 0932-0067 284:3<765 2011 284 404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer